Development and characterization of novel SSR markers in the endangered endemic species Ferula sadleriana

Applications in Plant Sciences

卷:8

Emese Meglécz¹  Gábor Sramkó²  Levente Laczkó²  Tamás Malkócs²  Judit Bereczki³  Péter Szövényi⁴ 

[1] Aix‐Marseille University Avignon University CNRS, IRD, IMBE Marseille France;

[2] Department of Botany University of Debrecen Debrecen Hungary;

[3] Department of Evolutionary Zoology and Human Biology University of Debrecen Debrecen Hungary;

[4] Institute of Systematic and Evolutionary Botany University of Zurich Zurich Switzerland;

关键词: Apiaceae;    conservation genetics;    endemic;    Ferula sadleriana;    microsatellite;    Umbelliferae;   

DOI  :  10.1002/aps3.11321

来源: DOAJ

【 摘 要 】

Premise Ferula sadleriana (Apiaceae) is a polycarpic, perennial herb with a very limited range and small populations. It is listed as “endangered” on the IUCN Red List of Threatened Species. Microsatellite markers can contribute to conservation efforts by allowing the study of the genetic structure of its shrinking populations. Methods and Results We used a microsatellite‐enriched library combined with an Illumina sequencing approach to develop simple sequence repeat markers in our target species. Out of 44 tested primer pairs, 22 provided specific products, and 13 showed heterologous amplification in the target species. Cross‐species amplification was achieved at 20 and 19 loci in two congeneric species, F. soongarica and F. tatarica, respectively. Conclusions The primers described here are the first tools that enable the population genetic characterization of F. sadleriana. Our results suggest a wider applicability in the genus Ferula.

【 授权许可】

Unknown