Inorganic Carbon Utilization of the Freshwater Red Alga Compsopogon coeruleus (Balbis) Montagne (Compsopogonaceae, Rhodophyta) Evaluated by in situ Measurement of Chlorophyll Fluorescence

Taiwania

卷:49

Liang-Chi Wang¹  Wei-Lung Wang¹  Shao-Lun Liu¹ 

[1]Department of Biology, National Changhua University of Education, Changhua 500, Taiwan.

关键词: Acetazolamide;    Carbonic anhydrase;    Fluorometer;    Inorganic carbon;    HEPES;    PAM;    Photosynthesis;   

DOI  :  10.6165/tai.2004.49(3).209

来源: DOAJ

【 摘 要 】

To explore the inorganic carbon utilization of the freshwater red alga Compsopogon coeruleus, photosynthetic rates in response to increasing of bicarbonate concentration, the addition of alkaline HEPES buffer (pH 8.8), acid HEPES buffer (pH 4.0) and the extracellular carbonic anhydrase inhibitor (acetazolamide, AZ), respectively, were examined in situ by using a submersible pulse amplitude modulated (PAM) fluorometer. Among the treatments, adding acid HEPES buffer significantly reduced photosynthetic rates of the alga, while others showed no effect. Accordingly, we concluded that C. coeruleus had less or no inorganic carbon (Ci) limitation in its natural habitat. The alga might have higher affinity for bicarbonate and directly uptake bicarbonate as main Ci source without the aid of extracellular carbonic anhydrase.

【 授权许可】

Unknown