Correlation of Glycated Haemoglobin with Protein Carbonyl Content as Biomarkers of Oxidative Stress in Type 2 Diabetes Mellitus

National Journal of Laboratory Medicine

卷:10

SHIKHA RAWAT¹  SUDEEP KUMAR²  ANJU MITTAL²  MANJU SHARMA³  AKSHAY SHARMA⁴ 

[1] Assistant Professor, Department of Biochemistry, Mulayam Singh Yadav Medical College, Hapur, Uttar Pradesh, India.;

[2] Assistant Professor, Department of Biochemistry, Muzaffarnagar Medical College, Muzaffarnagar, Uttar Pradesh, India.;

[3] Assistant Professor, Department of Biochemistry, Muzaffarnagar Medical College, Muzaffarnagar, Uttar Pradesh, India;

[4] Assistant Professor, Rama Medical College, Hospital and Research Centre, Hapur, Uttar Pradesh, India.;

关键词: dinitrophenylhydrazone;    fasting blood sugar;    hyperglycaemia;   

DOI  :  10.7860/NJLM/2021/45501:2470

来源: DOAJ

【 摘 要 】

Introduction: Diabetes is chronic disease which is characterisedby defect in insulin secretion and insulin action leading tohyperglycaemia.Aim: To detect protein carbonyl as oxidative stress marker inType 2 Diabetes Mellitus (T2DM).Materials and Methods: The present cross-sectional studywas conducted on 100 subjects (aged 40 to 70 years) out ofwhich 50 were T2DM and 50 were normal individuals. Serumwas tested for Fasting Blood Sugar (FBS) and GlycatedHaemoglobin (HbA1c) and Protein Carbonyl Content (PCC).Serum PCC was measured by spectrophotometric DNPH(dinitrophenylhydrazone) method of Reznick AZ and Packer L.Results: The protein carbonyl content, Fasting blood sugar andHbA1c were observed as positively correlated in type 2 diabetesmellitus as the levels of PCC, FBS and HbA1c were raised in T2DMas compared to controls.Conclusion: Increased HbA1c may be associated withgeneration of oxygen free radicals in the form of proteincarbonyls and decreased antioxidants in T2DM.

【 授权许可】

Unknown