Effect of acetate metabolism modulation on 2'-fucosyllactose production in engineered Escherichia coli

Biotechnology & Biotechnological Equipment

卷:35

Yuanfei Ge¹  Xiangsong Chen¹  Zhongkui Li¹  Yingxue Liao¹  Zhijian Ni¹  Jianming Yao¹  Jinyong Wu¹ 

[1] Institute of Plasma Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei;

关键词: 2'-fucosyllactose;    escherichia coli;    acetate;    culturing medium;    induction conditions;    glucose;   

DOI  :  10.1080/13102818.2021.1885996

来源: DOAJ

【 摘 要 】

2'-Fucosyllactose (2'-FL) is one of the most important oligosaccharides in human milk. In this study, a glucose–glycerol mixed carbon source and the deletion of the genes arcA and iclR were attempted for the first time in engineered Escherichia coli to improve 2'-FL production. The highest 2'-FL titre of 5.04 g/L, almost 1.4 times higher than that in the control strain, was obtained in the arcA mutant strain using shake-flask culture. In addition, the arcA mutant showed a significantly reduced accumulation of acetate. However, the deletion of iclR and the double deletion of arcA/iclR provided no benefit in terms of 2'-FL biosynthesis. Overall, these results suggest that deletion of arcA could efficiently increase the 2'-FL yield in E. coli and could lay a foundation for more efficient and larger-scale production. Moreover, in shake-flask culture under optimal conditions, the 2'-FL titer reached a maximum value of 7.14 g/L.

【 授权许可】

Unknown