Are miRNA-103, miRNA-107 and miRNA-122 Involved in the Prevention of Liver Steatosis Induced by Resveratrol?

Nutrients

卷:9

Alfredo Fernández-Quintela¹  Itziar Eseberri²  Jonatan Miranda³  María P. Portillo⁴  Marcela González⁵  Ana Gracia⁶ 

[1] [e-mail] (A.G.);

[2] [e-mail] (I.E.);

[3] [e-mail] (J.M.);

[4] [e-mail];

[5] [e-mail] (M.P.P.)2CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid 28029, Spain3Nutrition and Food Science, Faculty of Biochemistry and Biological Sciences, National University of Litoral, Santa Fe 3000, Argentina;

[6] 1Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria 01006, Spain;

关键词: miRNA-103;    miRNA-107;    miRNA-122;    steatosis;    liver;    resveratrol;    rat;   

DOI  :  10.3390/nu9040360

来源: DOAJ

【 摘 要 】

The aim of the present study was to determine whether the reduction in liver fat previously observed in our laboratory in a cohort of rats which had been fed an obesogenic diet was mediated by changes in the expression of microRNA (miRNA)-103-3p, miRNA-107-3p and miRNA-122-5p, which represent 70% of total miRNAs in the liver, as well as in their target genes. The expression of the three analysed miRNAs was reduced in rats treated with resveratrol. A reduction in sterol-regulatory element binding protein 1 (SREBP1) and an increase in carnitine palmitoyltransferase 1a (CPT1a) were observed in resveratrol-treated rats. No changes were found in fatty acid synthase (FAS). In cultured hepatocytes, SREBP1 protein was increased after the transfection of each miRNA. FAS protein expression was decreased after the transfection of miRNA-122-5p, and CPT1a protein was down-regulated by the over-expression of miRNA-107-3p. This study provides new evidences which show that srebf1 is a target gene for miRNA-103-3p and miRNA-107-3p, fasn a target gene for miRNA-122-5p and cpt1a a target gene for miRNA-107-3p. Moreover, the reduction in liver steatosis induced by resveratrol in rats fed an obesegenic diet is mediated, at least in part, by the increase in CPT1a protein expression and activity, via a decrease in miRNA-107-3p expression.

【 授权许可】

Unknown