Association of the Maternal Gut Microbiota/Metabolome with Cord Blood CCL17

Nutrients

卷:13

Yuki Shiko¹  Hiromi Tanabe²  Kenichi Sakurai²  Masahiro Watanabe²  Hideoki Fukuoka²  Chisato Mori²  Naoki Shimojo²  Ikumi Takashima³  Shingo Ochiai⁴  Fumiya Yamaide⁵  Taiji Nakano⁵  Yohei Kawasaki⁶  Yumiko Nakanishi⁷  Hiroshi Ohno⁷  Tamotsu Kato⁷  Naoko Taguchi-Atarashi⁷ 

[1] Biostatistics Section, Clinical Research Center, Chiba University Hospital, Chiba 260-8677, Japan;

[2] Center for Preventive Medical Sciences, Chiba University, Chiba 263-8522, Japan;

[3] Data Science Office, Clinical Research Promotion Center, University of Tokyo Hospital, Tokyo 113-8655, Japan;

[4] Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan;

[5] Department of Pediatrics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan;

[6] Faculty of Nursing, Japanese Red Cross College of Nursing, Tokyo 150-0012, Japan;

[7] Laboratory for Intestinal Ecosystem, RIKEN Center for Integrative Medical Sciences, Kanagawa 230-0045, Japan;

关键词: cord blood;    CCL17/TARC;    maternal gut microbiota;    short-chain fatty acids;   

DOI  :  10.3390/nu13082837

来源: DOAJ

【 摘 要 】

Chemokine (C-C motif) ligand 17 (CCL17) is a pro-allergic factor: high CCL17 levels in cord blood (CB) precede later allergic predisposition. Short-chain fatty acid (SCFA) treatment during pregnancy has been shown to protect mouse pups against allergic diseases. The maternal microbial metabolome during pregnancy may affect fetal allergic immune responses. We therefore examined the associations between CB CCL17 and gut SCFA levels in healthy pregnant Japanese women. CB CCL17 serum levels at birth, and maternal non-specific IgE levels in maternal sera at 32 weeks of gestation were measured. Maternal stool samples were collected at 12 (n = 59) and 32 (n = 58) weeks of gestation for gut microbiota analysis, based on barcoded 16S rRNA sequencing and metabolite levels. The CB CCL17 levels correlated negatively with butyrate concentrations and positively with isobutyrate at 12 weeks; CB CCL17 correlated positively with valerate and lactate at 32 weeks. Similarly, butyrate levels correlated negatively with maternal non-specific IgE levels, whereas the lactate concentration correlated positively with IgE levels. At 32 weeks, the Shannon diversity index (SDI) of Firmicutes and Proteobacteria correlated negatively with CB CCL17 levels, while those of the total microbiota correlated positively with the CB CCL17 levels. These metabolites may alter fetal immune responses. This study provides the first link between maternal metabolites during pregnancy and the risk of allergic diseases in human offspring.

【 授权许可】

Unknown