Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract

Electronic Journal of Biotechnology

卷:27

关键词: Biodiesel;    Biofuels;    Conversion of waste glycerol;    Dihydroxyacetone;    Fuel demand;    Glycerol dehydrogenase;    Glycerol waste fraction valorization;    Immobilized cell extract;    Renewable energy;    Substrate consumption;    Utilization;   

DOI  :  10.1016/j.ejbt.2017.03.003

来源: DOAJ

【 摘 要 】

Background: Depletion of petroleum resources has enforced the search for alternative sources of renewable energy. Introduction of biofuels into the market was expected to become a solution to this disadvantageous situation. Attempts to cover fuel demand have, however, caused another severe problem—the waste glycerol generated during biodiesel production at a concentration of approximately 10% w/w. This, in turn, prompted a global search for effective methods of valorization of the waste fraction of glycerol.Results: Utilization of the waste fraction at 48 h with an initial glycerol concentration of 30 g·L-1 and proceeding with 62% efficiency enabled the production of 9 g·L-1 dihydroxyacetone at 50% substrate consumption. The re-use of the immobilized biocatalyst resulted in a similar concentration of dihydroxyacetone (8.7 g·L-1) in two-fold shorter time, with an efficiency of 85% and lower substrate consumption (35%).Conclusions: The method proposed in this work is based on the conversion of waste glycerol to dihydroxyacetone in a reaction catalyzed by immobilized Gluconobacter oxydans cell extract with glycerol dehydrogenase activity, and it could be an effective way to convert waste glycerol into a valuable product.

【 授权许可】

Unknown