New Potentiometric Screen-Printed Platforms Modified with Reduced Graphene Oxide and Based on Man-Made Imprinted Receptors for Caffeine Assessment

Polymers

卷:14

Hisham S. M. Abd-Rabboh¹  Ayman H. Kamel²  Abdulrahman A. Almehizia³  Abdel El-Galil E. Amr³  Ahmed M. Naglah³ 

[1] Chemistry Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha 61413, Saudi Arabia;

[2] Department of Chemistry, Faculty of Science, Ain Shams University, Cairo 11566, Egypt;

[3] Pharmaceutical Chemistry Department, College of Pharmacy, Drug Exploration and Development Chair (DEDC), King Saud University, Riyadh 11451, Saudi Arabia;

关键词: solid-contact ISEs;    caffeine;    molecularly imprinted polymers (MIPs);    screen printed;    potentiometry;   

DOI  :  10.3390/polym14101942

来源: DOAJ

【 摘 要 】

Caffeine is a psychoactive drug that is administered as a class II psychotropic substance. It is also considered a component of analgesics and cold medicines. Excessive intake of caffeine may lead to severe health damage or drug addiction problems. The assessment of normal caffeine consumption from abusive use is not conclusive, and the cut-off value for biological samples has not been established. Herein, new cost-effective and robust all-solid-state platforms based on potentiometric transduction were fabricated and successfully utilized for caffeine assessment. The platforms were modified with reduced graphene oxide (rGO). Tailored caffeine-imprinted polymeric beads (MIPs) based on methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) were prepared, characterized, and used as recognition receptors in the presented potentiometric sensing devices. In 50 mM MES buffer, the sensors exhibited a slope response of 51.2 ± 0.9 mV/decade (n = 6, R² = 0.997) over the linear range of 4.5 × 10⁻⁶–1.0 × 10⁻³ M with a detection limit of 3.0 × 10⁻⁶ M. They exhibited fast detection of caffeinium ions with less than 5 s response time (<5 s). The behavior of the presented sensors towards caffeinium ions over many common organic and inorganic cations was evaluated using the modified separate solution method (MSSM). Inter-day and intra-day precision for the presented analytical device was also evaluated. Successful applications of the presented caffeine sensors for caffeine determination in commercial tea and coffee and different pharmaceutical formulations were carried out. The data obtained were compared with those obtained by the standard liquid chromatographic approach. The presented analytical device can be considered an attractive tool for caffeine determination because of its affordability and vast availability, particularly when combined with potentiometric detection.

【 授权许可】

Unknown