Genetic Effects of Polymorphisms in Myogenic Regulatory Factors on Chicken Muscle Fiber Traits

Asian-Australasian Journal of Animal Sciences

卷:28

Xiao-Ling Zhao¹  Hua-Dong Yin²  Yi-Ping Liu²  Di-Yan Li²  Ying Qing²  Qing Zhu²  Yan Wang² 

[1]College of Animal Science and Technology, Sichuan Agricultural University, Ya’an, Sichuan, 625014, .China

[2]|

关键词: Myogenic Regulatory Factors [MRFs];    Polymorphism;    Chicken;    Muscle Fiber Traits;   

DOI  :  10.5713/ajas.14.0753

来源: DOAJ

【 摘 要 】

The myogenic regulatory factors is a family of transcription factors that play a key role in the development of skeletal muscle fibers, which are the main factors to affect the meat taste and texture. In the present study, we performed candidate gene analysis to identify single-nucleotide polymorphisms in the MyoD, Myf5, MyoG, and Mrf4 genes using polymerase chain reaction-single strand conformation polymorphism in 360 Erlang Mountain Chickens from three different housing systems (cage, pen, and free-range). The general linear model procedure was used to estimate the statistical significance of association between combined genotypes and muscle fiber traits of chickens. Two polymorphisms (g.39928301T>G and g.11579368C>T) were detected in the Mrf4 and MyoD gene, respectively. The diameters of thigh and pectoralis muscle fibers were higher in birds with the combined genotypes of GG-TT and TT-CT (p<0.05). Moreover, the interaction between housing system and combined genotypes has no significant effect on the traits of muscle fiber (p>0.05). Our findings suggest that the combined genotypes of TT-CT and GG-TT might be advantageous for muscle fiber traits, and could be the potential genetic markers for breeding program in Erlang Mountain Chickens.

【 授权许可】

Unknown