Detection of icaA/icaD Genes and Biofilm Formation among Clinical Isolates of Staphylococcus aureus from Shiraz, Iran

Journal of Medical Bacteriology

卷:4

Mohammad Motamedifar¹  Mehrdad Zalipour²  Jamal Sarvari²  Reza Khashei²  Hadi Sedigh Ebrahim-Saraie² 

[1] Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, IranShiraz HIV/AIDS Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.;

[2] Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.;

关键词: Biofilm;    Staphylococcus aureus;    Congo red agar;    icaA;    icaD;   

DOI  :  

来源: DOAJ

【 摘 要 】

Introduction: Biofilm producing Staphylococcus aureus is known as one of the majorcausative agents of infections, failure of implanted devices and persistent infectionamong hospitalized patients. The aim of the present study was to determine thefrequency of biofilm producing S. aureus isolates amongst the clinical specimens.Methods: This cross-sectional study was conducted during 2012 to 2013 in twoteaching hospitals in Shiraz, southwest of Iran. Totally, 345 S. aureus isolates fromvarious clinical specimens were included. Biofilm producing isolates werephenotypically detected using Congo Red Agar (CRA) and genotypically by PCRassay for the icaA and icaD genes.Results: Of the 345 S. aureus isolates, 42.3% were methicillin-resistant S. aureus(MRSA) and subsequently 57.7% were methicillin susceptible isolates. The results ofCRA plates showed that 77 (52.7%) and 74 (37.2%) of MRSA and MSSA werebiofilm producing isolates. The frequency of icaA/D genes among MRSA and MSSAisolates was 127 (87%) and 167 (83.9%), respectively.Conclusion: Such a high rate of icaA/D harboring S. aureus among clinical isolatessuggest the risk for establishing persistent infections in the hospital settings.

【 授权许可】

Unknown