Can MMP-9 be a Prognosticator Marker for Oral Squamous Cell Carcinoma?

Journal of Clinical and Diagnostic Research

卷:10

Shiva Kumar Basu¹  Manish Kumar²  Betina Chandolia³ 

[1] Professor and Head, Department of Oral Pathology & Microbiology, M.M. College of Dental Sciences and Research, Ambala, Haryana, India.;

[2] Senior Lecturer, Department of Oral Pathology & Microbiology, M.M. College of Dental Sciences and Research, Ambala, Haryana, India.;

[3] Senior Lecturer, Department of Oral Pathology and Microbiology, M.M. College of Dental Sciences and Research, Ambala, Haryana, India.;

关键词: immunohistochemistry;    invasion and metastasis;    oral cancer;    oral epithelial dysplasia;    tumour progression;   

DOI  :  10.7860/JCDR/2016/14128.7034

来源: DOAJ

【 摘 要 】

Introduction: Invasion and metastasis of malignant tumoursseverely endanger the life of cancer patients. Squamous cellcarcinoma is one of the commonly found malignancies in theoral cavity and its survival rate has not improved from past fewdecades. Since an important risk factor for oral squamous cellcarcinoma is the presence of epithelial dysplasia, it is necessaryto check the presence of a prognosticator marker in both of them.As matrix metalloproteinase’s (MMP’s) are involved in degradationof type IV collagen, which are one of the important components ofextracellular matrix components which play a relevant role in severalsteps of tumour progression such as invasion and metastasis.We have studied MMP-9 expression to evaluate its prognosticpotential in oral cancers as well as oral epithelial dysplasia alongwith tissues of normal oral epithelium.Materials and Methods: The expression was examined usingimmunohistochemistry procedure with MMP-9 in 100 samplesincluding cases of epithelium from normal oral mucosa, oraldysplastic lesions and oral squamous cell carcinoma. One set offormalin fixed, paraffin embedded sections of the three categorieswere stained by haematoxylin and eosin. The sections were thenevaluated under microscope. Data was examined for statisticalsignificance using SPSS 13.0 by Mann-Whitney Test and KruskalWallis Test.Results: With MMP-9 gain of expression was noted from Controlgroup to oral squamous cell carcinoma. Cytoplasmic stainingwas seen with MMP-9. Statistically highly significant differenceswere seen between oral epithelial dysplasia and oral squamouscell carcinoma and statistically significant differences were foundbetween the control group and the oral squamous cell carcinomagroup.Conclusion: This study suggested that oral squamous cellcarcinoma shows higher MMP-9 expression as compared to oralepithelial dysplasia followed by epithelium from normal oral mucosa.However, no correlation was found among the histological gradesof oral squamous cell carcinoma.

【 授权许可】

Unknown