Two Cladosporium Fungi with Opposite Functions to the Chinese White Wax Scale Insect Have Different Genome Characters

Journal of Fungi

卷:8

Shu-Hui Yu¹  Hong-Ping Zhang¹  Jin-Yang Zhang²  Jia-Qi An³  Zuo-Yi Fu³  Wei Liu³  Pu Yang³ 

[1] College of Agriculture and Life Sciences, Kunming University, Kunming 650214, China;

[2] Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China;

[3] Institute of Highland Forest Science, Chinese Academy of Forestry, Kunming 650224, China;

关键词: Chinese white wax scale insect;    Cladosporium;    genome;    endogensis;    pathogen;    adaptation;   

DOI  :  10.3390/jof8030286

来源: DOAJ

【 摘 要 】

Insects encounter infection of microorganisms, and they also harbor endosymbiosis to participate in nutrition providing and act as a defender against pathogens. We previously found the Chinese white wax scale insect, Ericerus pela, was infected and killed by Cladosporium sp. (pathogen). We also found it harbored Cladosporium sp. (endogensis). In this study, we cultured these two Cladosporium fungi and sequenced their genome. The results showed Cladosporium sp. (endogensis) has a larger genome size and more genes than Cladosporium sp. (pathogen). Pan-genome analysis showed Cladosporium sp. (endogensis)-specific genes enriched in pathways related to nutrition production, such as amino acid metabolism, carbohydrate metabolism, and energy metabolism. These pathways were absent in that of Cladosporium sp. (pathogen). Gene Ontology analysis showed Cladosporium sp. (pathogen)-specific genes enriched in the biosynthesis of asperfuranone, emericellamide, and fumagillin. These terms were not found in that of Cladosporium sp. (endogensis). Pathogen Host Interactions analysis found Cladosporium sp. (endogensis) had more genes related to loss of pathogenicity and reduced virulence than Cladosporium sp. (pathogen). Cytotoxicity assay indicated Cladosporium sp. (pathogen) had cytotoxicity, while Cladosporium sp. (endogensis) had no cytotoxicity. These characters reflect the adaptation of endosymbiosis to host-restricted lifestyle and the invader of the entomopathogen to the host.

【 授权许可】

Unknown