Antibacterial activities of Calendula officinalis callus extract

International Journal of Secondary Metabolite

卷:4

关键词: calendula officinalis;    callus;    antibacterial activity;    bap;    naa;   

DOI  :  10.21448/ijsm.372108

来源: DOAJ

【 摘 要 】

The purpose of this study was to determine the antibacterialactivity of C. officinalis callus derivedfrom cotyledon explants. Cotyledons excised from in vitro germinated seedlings were used as explants. Explants were transferredon MS medium supplemented with benzil amino purine (BAP; 2 mg l-1), α-naphthalene-aceticacid (NAA; 2 mg l-1) for callus studies. The cultures were maintainedon the same media compositions and were sub-cultured at an interval of 4 weeks.Callus cultures were harvested at the end of the 16th week. Calli were dried at40̊ C in the dark for antimicrobial studies. Calendula officinalis callus extracts were tested for their antibacterialactivities by using agar well diffusion method. Ethanol and chloroform extractsfrom these plants were assayed against nine bacteria species (Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, Salmonella typhimurium CCM 5445, Proteus vulgaris ATCC 6896, Enterococcus faecalis ATCC 29212, Enterobacter cloacae ATCC 13047, and Kocuria rhizophila ATCC 9341). The test antibioticspenicillin G, novobiocin, amphicillin, chloramphenicol and erythromycin were usedfor comparison. Callus formation was observed at the end of the 5th week on cotyledonexplants. C. officinalis callus extractsshowed 38 mm inhibition zone against S. aureus,and chloroform extracts showed 32 mm inhibition zone against B. cereus. These results are very close tothe test antibiotics used and C. officinalisis found more effective on gram positive bacteria.

【 授权许可】

Unknown