Biocompatibility Study of a New Dental Cement Based on Hydroxyapatite and Calcium Silicates: Focus on Liver, Kidney, and Spleen Tissue Effects

International Journal of Molecular Sciences

卷:22

Damjana Drobne¹  Dijana Trišić²  Smiljana Paraš³  Olivera Mitrović Ajtić⁴  Božana Čolović⁵  Đorđe Antonijević⁵  Vukoman Jokanović⁵ 

[1] Biotechnical Faculty, University of Ljubljana, 1 000 Ljubljana, Slovenia;

[2] Faculty of Dental Medicine, University of Belgrade, 11 000 Belgrade, Serbia;

[3] Faculty of Science and Mathematics, University of Banja Luka, 51 000 Banja Luka, Republic of Srpska, Bosnia and Herzegovina;

[4] Institute for Medical Research, University of Belgrade, 11 000 Belgrade, Serbia;

[5] Vinča Institute of Nuclear Sciences, University of Belgrade, 11 000 Belgrade, Serbia;

关键词: biocompatibility;    calcium silicates;    dental cement;    hydroxyapatite;    subchronic toxicity;   

DOI  :  10.3390/ijms22115468

来源: DOAJ

【 摘 要 】

The effects of a new material based on hydroxyapatite and calcium silicates, named ALBO-MPCA, were investigated on the liver, kidney and spleen. The material was administrated orally for 120 days in an in vivo model in Wistar rats, and untreated animals served as a control. Hematological and biochemical blood parameters were analyzed. Qualitative histological analysis of tissues, change in mitotic activity of cells, and histological characteristics was conducted, as well as quantitative stereological analysis of parenchymal cells, blood sinusoids, and connective tissues. Additionally, the protein expressions of Ki67 and CD68 markers were evaluated. Histological analysis revealed no pathological changes after the tested period. It showed the preservation of the architecture of blood sinusoids and epithelial cells and the presence of mitosis. Additionally, the significantly increased number of the Ki67 in the presence of ALBO-MPCA confirmed the proliferative effect of the material noticed by stereological analysis, while immunoreactive CD68 positive cells did not differ between groups. The study showed non-toxicity of the tested material based on the effects on the hematological, biochemical, and observed histological parameters; in addition, it showed evidence of its biocompatibility. These results could be the basis for further steps toward the application of tested materials in endodontics.

【 授权许可】

Unknown