Hepatoprotective Effects of Lactobacillus on Carbon Tetrachloride-Induced Acute Liver Injury in Mice

International Journal of Molecular Sciences

卷:19

Jing Zhang¹  Zhennai Yang²  Xiaoyong Chen²  Jianfei Mu³  Xin Zhao³  Ruokun Yi⁴ 

[1] Business University (BTBU), Beijing 102488, China;

[2] Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology &

[3] Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China;

[4] Department of Environmental and Quality Inspection, Chongqing Chemical Industry Vocational College, Chongqing 401220, China;

关键词: Lactobacillus fermentum;    Lactobacillus plantarum;    hepatoprotective effect;    CCl4;   

DOI  :  10.3390/ijms19082212

来源: DOAJ

【 摘 要 】

The aim of this study was to investigate and compare the effects of heat-killed and live Lactobacillus on carbon tetrachloride (CCl4)-induced acute liver injury mice. The indexes evaluated included liver pathological changes, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) in the serum, related gene expression (IL-1β, TNF-α, Bcl-2, and Bax), and related proteins levels (Bax, Bcl-2, Caspase 3, and NF-κB p65). Compared with the model group, the results indicated that the levels of ALT, AST, and MDA in the serum, the expression levels of IL-1β, TNF-α, and Bax, and the protein levels of Bax, Caspase 3, and NF-κB p65 significantly decreased, and the pathologic damage degree all significantly reduced after live Lactobacillus fermentum (L-LF) and live Lactobacillus plantarum (L-LP) treatment. Additionally, the levels of SOD and GSH in the serum, the gene expression of Bcl-2, and the protein level of Bcl-2 significantly increased after L-LF and L-LP treatment. Although HK-LF and HK-LP could also have obvious regulating effects on some of the evaluated indexes (ALT, AST, the expression levels of TNF-α and Bax, and the protein level of Bcl-2) and play an important role in weakening liver damage, the regulating effects of L-LF or L-LP on these indexes were all better compared with the corresponding heat-killed Lactobacillus fermentum (HK-LF) and heat-killed Lactobacillus plantarum (HK-LP). Therefore, these results suggested that LF and LP have an important role in liver disease.

【 授权许可】

Unknown