Inhalation of marijuana affects Drosophila heart function

Biology Open

卷:8

Paola Ferrero¹  Ivana M. Gómez¹  Carlos A. Valverde¹  Manuela Santalla¹  Maia A. Rodríguez¹  Daniela Sedán²  Dario Andrinolo²  Jorge E. Colman Lerner³  George Kassis⁴  J. Oswaldo Aranda⁵ 

[1] Centro de Investigaciones Cardiovasculares ‘Dr. Horacio E. Cingolani’, Facultad de Ciencias Médicas, UNLP, La Plata 1900, Argentina;

[2] Centro de Investigaciones del medio Ambiente Facultad de Ciencias Exactas, CCT La Plata-UNLP, La Plata 1900, Argentina;

[3] Centro de Investigación y Desarrollo en Ciencias Aplicadas Facultad de Ciencias Exactas, CCT La Plata-UNLP-CICPBA, La Plata 1900, Argentina;

[4] Medicalbit, La Plata 1900, Argentina;

[5] Programa Ambiental de Extensión Universitaria. Facultad de Ciencias Exactas–UNLP, La Plata 1900, Argentina;

关键词: Drosophila;    Cannabis;    Cardiac;    THC;    CBD;    Calcium;   

DOI  :  10.1242/bio.044081

来源: DOAJ

【 摘 要 】

We investigated the effect of inhalation of vaporized marijuana on cardiac function in Drosophila melanogaster, a suitable genetic model for studying human diseases. Adult flies were exposed to marijuana for variable time periods and the effects on cardiac function were studied. Short treatment protocol incremented heart-rate variability. Contractility was augmented only under prolonged exposure to cannabis and it was associated with incremented calcium transient within cardiomyocytes. Neither the activity of the major proteins responsible for calcium handling nor the calcium load of the sarcoplasmic reticulum were affected by the cannabis treatment. The observed changes manifested in the cardiomyocytes even in the absence of the canonical cannabinoid receptors described in mammals. Our results are the first evidence of the in vivo impact of phytocannabinoids in D. melanogaster. By providing a simple and affordable platform prior to mammalian models, this characterization of cardiac function under marijuana exposure opens new paths for conducting genetic screenings using vaporized compounds. This article has an associated First Person interview with the first author of the paper.

【 授权许可】

Unknown