Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15

Frontiers in Bioengineering and Biotechnology

卷:9

Yanqing Li¹  Jikun Xia²  Jiakun Xu³  Fang Wang³  Hengmin Miao³  Xiaochun Sun⁴  Maosheng Li⁵  Jiao Li⁶ 

[1] College of Food Science and Technology, Shanghai Ocean University, Shanghai, China;

[2] College of Marine Science and Biological Engineering, Qingdao University of Science andTechnology, Qingdao, China;

[3] Key Lab of Sustainable Development of Polar Fisheries, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China;

[4] Marine Science Research Institute of Shandong Province, Qingdao, China;

[5] School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China;

[6] School of Materials Science and Engineering, Shandong University of Technology, Zibo, China;

关键词: myoglobin;    SBA-15;    immobilization;    catalytic activity;    reusability;   

DOI  :  10.3389/fbioe.2021.827552

来源: DOAJ

【 摘 要 】

A series of stable mesoporous silica sieves (SBA-15) with different pore sizes (9.8, 7.2, and 5.5 nm) were synthesized using a hydrothermal method. The resulting mesoporous material was then utilized for protein immobilization using myoglobin (Mb) as the target protein. The effects of pore size and adsorption methods on the immobilization efficiency of Mb in a mesoporous material were studied. The SBA-15 with a pore size of 7.2 nm showed the best loading capacity, reaching 413.8 mg/g. The SBA-15 with a pore size of 9.8 nm showed the highest retained catalytic ability (92.36%). The immobilized enzyme was more stable than the free enzyme. After seven consecutive assay cycles, Mb adsorbed by SBA-15 (Mb/SBA-15) and Mb adsorbed by SBA-15 and crosslinked with glutaraldehyde (Mb/G/SBA-15) retained 36.41% and 62.37% of their initial activity, respectively.

【 授权许可】

Unknown