Deciphering the Evolutionary History of Arowana Fishes (Teleostei, Osteoglossiformes, Osteoglossidae): Insight from Comparative Cytogenomics

International Journal of Molecular Sciences

卷:20

Wagner Franco Molina¹  Luiz Antonio Carlos Bertollo²  Cassia Fernanda Yano²  Marcelo de Bello Cioffi²  Fernando Henrique Santos de Souza²  Ezequiel Aguiar de Oliveira²  Terumi Hatanaka²  Manolo Fernandez Perez²  Tariq Ezaz³  Peter Unmack³  Ahmed Basheer Hamid Al-Rikabi⁴  Thomas Liehr⁴  Eliana Feldberg⁵  Patrik Viana⁵  Zuzana Majtánová⁶  Alexandr Sember⁶  Petr Ráb⁶  Sebastien Lavoué⁷  Alongklod Tanomtong⁸ 

[1] Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN 59078-970, Brazil;

[2] Departamento de Genética e Evolução, Universidade Federal de São Carlos (UFSCar), São Carlos, SP 13565-090, Brazil;

[3] Institute for Applied Ecology, University of Canberra, Canberra, ACT 2617, Australia;

[4] Institute of Human Genetics, University Hospital Jena, 07747 Jena, Germany;

[5] Instituto Nacional de Pesquisas da Amazônia, Coordenação de Biodiversidade, Laboratório de Genética Animal, Petrópolis, Manaus, AM 69077-000, Brazil;

[6] Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 27721 Liběchov, Czech Republic;

[7] School of Biological Sciences, Universiti Sains Malaysia, Penang 11800, Malaysia;

[8] Toxic Substances in Livestock and Aquatic Animals Research Group, KhonKaen University, Muang, KhonKaen 40002, Thailand;

关键词: biogeography;    DArTseq;    evolution;    genetic diversity;    Gondwana;   

DOI  :  10.3390/ijms20174296

来源: DOAJ

【 摘 要 】

Arowanas (Osteoglossinae) are charismatic freshwater fishes with six species and two genera (Osteoglossum and Scleropages) distributed in South America, Asia, and Australia. In an attempt to provide a better assessment of the processes shaping their evolution, we employed a set of cytogenetic and genomic approaches, including i) molecular cytogenetic analyses using C- and CMA₃/DAPI staining, repetitive DNA mapping, comparative genomic hybridization (CGH), and Zoo-FISH, along with ii) the genotypic analyses of single nucleotide polymorphisms (SNPs) generated by diversity array technology sequencing (DArTseq). We observed diploid chromosome numbers of 2n = 56 and 54 in O. bicirrhosum and O. ferreirai, respectively, and 2n = 50 in S. formosus, while S. jardinii and S. leichardti presented 2n = 48 and 44, respectively. A time-calibrated phylogenetic tree revealed that Osteoglossum and Scleropages divergence occurred approximately 50 million years ago (MYA), at the time of the final separation of Australia and South America (with Antarctica). Asian S. formosus and Australian Scleropages diverged about 35.5 MYA, substantially after the latest terrestrial connection between Australia and Southeast Asia through the Indian plate movement. Our combined data provided a comprehensive perspective of the cytogenomic diversity and evolution of arowana species on a timescale.

【 授权许可】

Unknown