An Integrated Mass Spectrometry-Based Glycomics-Driven Glycoproteomics Analytical Platform to Functionally Characterize Glycosylation Inhibitors

Molecules

卷:27

Carlito B. Lebrilla¹  Qingwen Zhou¹  Gladys C. Completo²  Ruel C. Nacario²  Michael Russelle S. Alvarez²  Sheryl Joyce B. Grijaldo²  Jomar F. Rabajante³  Francisco M. Heralde⁴ 

[1] Department of Chemistry, University of California Davis, Davis, CA 95616, USA;

[2] Institute of Chemistry, College of Arts and Sciences, University of the Philippines Los Baños, Los Baños 4031, Philippines;

[3] Institute of Mathematical Sciences and Physics, College of Arts and Sciences, University of the Philippines Los Baños, Los Baños 4031, Philippines;

[4] Lung Center of the Philippines, Quezon City 1100, Philippines;

关键词: glycomics;    glycoproteomics;    glycosylation;    proteomics;    in silico docking;    network pharmacology;   

DOI  :  10.3390/molecules27123834

来源: DOAJ

【 摘 要 】

Cancer progression is linked to aberrant protein glycosylation due to the overexpression of several glycosylation enzymes. These enzymes are underexploited as potential anticancer drug targets and the development of rapid-screening methods and identification of glycosylation inhibitors are highly sought. An integrated bioinformatics and mass spectrometry-based glycomics-driven glycoproteomics analysis pipeline was performed to identify an N-glycan inhibitor against lung cancer cells. Combined network pharmacology and in silico screening approaches were used to identify a potential inhibitor, pictilisib, against several glycosylation-related proteins, such as Alpha1-6FucT, GlcNAcT-V, and Alpha2,6-ST-I. A glycomics assay of lung cancer cells treated with pictilisib showed a significant reduction in the fucosylation and sialylation of N-glycans, with an increase in high mannose-type glycans. Proteomics analysis and in vitro assays also showed significant upregulation of the proteins involved in apoptosis and cell adhesion, and the downregulation of proteins involved in cell cycle regulation, mRNA processing, and protein translation. Site-specific glycoproteomics analysis further showed that glycoproteins with reduced fucosylation and sialylation were involved in apoptosis, cell adhesion, DNA damage repair, and chemical response processes. To determine how the alterations in N-glycosylation impact glycoprotein dynamics, modeling of changes in glycan interactions of the ITGA5–ITGB1 (Integrin alpha 5-Integrin beta-1) complex revealed specific glycosites at the interface of these proteins that, when highly fucosylated and sialylated, such as in untreated A549 cells, form greater hydrogen bonding interactions compared to the high mannose-types in pictilisib-treated A549 cells. This study highlights the use of mass spectrometry to identify a potential glycosylation inhibitor and assessment of its impact on cell surface glycoprotein abundance and protein–protein interaction.

【 授权许可】

Unknown