Microarray Glycan Profiling Reveals Algal Fucoidan Epitopes in Diverse Marine Metazoans

Frontiers in Marine Science

卷:4

Cécile Hervé¹  Bodil Jørgensen²  Armando A. Salmeán²  Jozef Mravec²  William G. T. Willats³ 

[1] Centre National de la Recherche Scientifique/UPMC, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074Roscoff, France;

[2] Department of Plant and Environmental Sciences, University of CopenhagenFrederiksberg, Denmark;

[3] School of Agriculture, Food and Rural Development, Newcastle UniversityNewcastle upon Tyne, United Kingdom;

关键词: fucoidans;    microarray profiling;    structural polysaccharides;    anti-glycan antibodies;    immunolocalisation;    sponges;   

DOI  :  10.3389/fmars.2017.00293

来源: DOAJ

【 摘 要 】

Despite the biological importance and pharmacological potential of glycans from marine organisms, there are many unanswered questions regarding their distribution, function, and evolution. Here we describe microarray-based glycan profiling of a diverse selection of marine animals using antibodies raised against fucoidan isolated from a brown alga. We demonstrate the presence of two fucoidan epitopes in six animals belonging to three phyla including Porifera, Molusca, and Chordata. We studied the spatial distribution of these epitopes in Cliona celata (“boring sponge”) and identified their restricted localization on the surface of internal chambers. Our results show the potential of high-throughput screening and probes commonly used in plant and algal cell wall biology to study the diversity and distribution of glycan structures in metazoans.

【 授权许可】

Unknown