Electrospun Fibers Derived from Peptide Coupled Amphiphilic Copolymers for Dorsal Root Ganglion (DRG) Outgrowth

Gels

卷:7

Jixiang Zhu¹  Wensheng Lin¹  Shuo Tang²  Xingwu Zhou³  Zhu Liu⁴  Ming Lu⁴  Si Qiu⁴  Na Qiang⁴ 

[1] Department of Biomedical Engineering, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 511436, China;

[2] Department of Orthopaedics, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 517000, China;

[3] Department of Pharmaceutical Sciences, University of Michigan, Ann Arbor, MI 48109, USA;

[4] School of Chemistry and Materials Engineering, Huizhou University, Huizhou 516007, China;

关键词: electrospun;    functional monomer;    isoleucine-lysine-valine-alanine-valine;    dorsal root ganglion;   

DOI  :  10.3390/gels7040196

来源: DOAJ

【 摘 要 】

Developing scaffolds with appropriate mechanical/structural features as well as tunable bioactivities are indispensable in the field of tissue engineering. This study focused on one such attempt to electrospin the copolymer of L-lactic acid (L-LA) and functional monomer (3(S)- [(benzyloxycarbony)methyl]-1,4-dioxane-2,5-dione, BMD) with small peptide modifications for the purpose of neural tissue engineering. Scanning Electron Microscopy (SEM) micrographs showed fabricated electrospun copolymer as porous and uniform nanofibrous materials with diameter in the range of 800–1000 nm. In addition, the modified scaffolds displayed a lower contact angle than poly(L-lactide) (PLLA) indicating higher hydrophilicity. To further incorporate the bioactive functions, the nanofibers were chemically coupled with small peptide (isoleucine-lysine-valine-alanine-valine, IKVAV). The incorporation of IKVAV onto the electrospun fiber was confirmed by X-ray photoelectron spectroscopy (XPS) and such incorporation did not affect the surface morphology or fiber diameters. To demonstrate the potential of applying the designed scaffolds for nerve regeneration, dorsal root ganglion (DRG) neurons were cultured on the nanofibers to examine the impact on neurite outgrowth of DRGs. The results indicated that the fabricated nanofibrous matrix with small peptide might be a potential candidate for neural tissue engineering.

【 授权许可】

Unknown