Identification of Arenin, a Novel Kunitz-Like Polypeptide from the Skin Secretions of Dryophytes arenicolor

International Journal of Molecular Sciences

卷:19

PatriciaL. Conway¹  Jørgen Schlundt¹  Aida Serra²  SiuKwan Sze²  Jorge Benavides³  Jesús Hernández-Pérez³ 

[1] Nanyang Technological University Food Technology Centre (NAFTEC), Nanyang Technological University (NTU), Singapore 637459, Singapore;

[2] School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, Singapore 637551, Singapore;

[3] School of Engineering and Science, Tecnologico de Monterrey, Av. Eugenio Garza Sada 2501 Sur, C.P. 64849 Monterrey, N.L., Mexico;

关键词: amphibians;    skin secretions;    Dryophytes arenicolor;    Kunitz-like polypeptide;    cytotoxicity;   

DOI  :  10.3390/ijms19113644

来源: DOAJ

【 摘 要 】

Amphibian skin secretions are enriched with complex cocktails of bioactive molecules such as proteins, peptides, biogenic amines, alkaloids guanidine derivatives, steroids and other minor components spanning a wide spectrum of pharmacological actions exploited for centuries in folk medicine. This study presents evidence on the protein profile of the skin secretions of the canyon tree frog, Dryophytes arenicolor. At the same time, it presents the reverse-phase liquid chromatography isolation, mass spectrometry characterization and identification at mRNA level of a novel 58 amino acids Kunitz-like polypeptide from the skin secretions of Dryophytes arenicolor, arenin. Cell viability assays performed on HDFa, CaCo2 and MCF7 cells cultured with different concentrations of arenin showed a discrete effect at low concentrations (2, 4, 8 and 16 µg/mL) suggesting a multi-target interaction in a hormetic-like dose-response. Further work is required to investigate the mechanisms underlying the variable effect on cell viability produced by different concentrations of arenin.

【 授权许可】

Unknown