Parkin and synphilin-1 isoform expression changes in Lewy body diseases

Neurobiology of Disease

卷:26

Cristina Carrato¹  Katrin Beyer²  José L. Mate²  Isidro Ferrer²  Jordi Humbert²  Aurelio Ariza³ 

[1] Corresponding author. Fax: [phone].;

[2] Departments of Pathology, Hospital Germans Trias i Pujol, Autonomous University of Barcelona, 08916 Badalona, Spain;

[3] Institute of Neuropathology, Hospital de Bellvitge, L’Hospitalet de Llobregat, Spain;

关键词: Dementia with Lewy bodies;    Parkinson disease;    Lewy body variant of Alzheimer disease;    Differential isoform expression;    Transcript variant;   

DOI  :  

来源: DOAJ

【 摘 要 】

Alternative splicing gives rise to at least seven parkin and eight synphilin-1 isoforms. Since both parkin and synphilin-1 have been involved in Lewy body (LB) formation, we decided to explore whether their isoforms are differentially expressed in LB diseases. With this aim, we studied relative mRNA expression levels of parkin and synphilin-1 isoforms in the frontal cortices of patients with dementia with LBs, the LB variant of Alzheimer’s disease and Parkinson’s disease and compared the findings with those obtained from Alzheimer’s disease patients and control individuals. Duplex real-time PCR reactions, with beta-actin as internal standard, were carried out in a LightCycler. mRNA expression levels of parkin and synphilin-1 isoforms were seen to be specifically altered in each of the LB diseases studied. These findings suggest that parkin and synphilin-1 isoform expression changes play a significant role in the pathogenesis of LB diseases.

【 授权许可】

Unknown