The effect of anesthetic dose on the motor response induced by low-intensity pulsed ultrasound stimulation

BMC Neuroscience

卷:19

Jiaqing Yan¹  Xingran Wang²  Yi Yuan²  Xiaoli Li³ 

[1] College of Electrical and Control Engineering, North China University of Technology;

[2] Institute of Electrical Engineering, Yanshan University;

[3] State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University;

关键词: LIPUS;    Anesthetic dose;    Mouse;    Motion response;   

DOI  :  10.1186/s12868-018-0476-2

来源: DOAJ

【 摘 要 】

Abstract Background Low-intensity pulsed ultrasound stimulation (LIPUS) has been proven to be a noninvasive method with high spatial resolution and deep penetration. Previous studies have qualitatively demonstrated that the electromyographic response caused by LIPUS in the mouse motor cortex is affected by the anesthetic state of the mice. However, the quantitative relationship between motor response and anesthetic dose remains unclear. Results Experimental results show that the success rate decreases stepwise as the isoflurane concentration/mouse weight ratio increases (ratios: [0.004%/g, 0.01%/g], success rate: ~ 90%; [0.012%/g, 0.014%/g], ~ 40%; [0.016%/g, 0.018%/g], ~ 7%; 0.024%/g, 0). The latency and duration of EMG increase significantly when the ratio is more than 0.016%/g. Compared with that at ratios from 0.004 to 0.016%/g, normalized EMG amplitude decreases significantly at ratios of 0.018%/g and 0.020%/g. Conclusions Quantitative calculations indicate that the anesthetic dose has a significant regulatory effect on the motor response of mice during LIPUS. Our results have guiding significance for the selection of the anesthetic dose for LIPUS in mouse motor cortex experiments.

【 授权许可】

Unknown