Single Cell Raman Spectroscopy Deuterium Isotope Probing for Rapid Antimicrobial Susceptibility Test of Elizabethkingia spp.

Frontiers in Microbiology

卷:13

Yizhi Song¹  Na He¹  Kaicheng Lin¹  Chaoyang Tong²  Zhenju Song²  Wenhong Zhang³  Danfeng Shen⁴  Xinrong Lu⁴  Lin Zou⁴  Li Chen⁴  Yanwen Chen⁵  Shaoxing Zhang⁵  Guiqin Sun⁵  Shuying Yuan⁵  Ruijie Liu⁵ 

[1] CAS Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, China;

[2] Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;

[3] Department of Infectious Diseases, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China;

[4] Department of Medical Microbiology and Parasitology, Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China;

[5] School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou, China;

关键词: Elizabethkingia spp.;    single cell Raman spectroscopy deuterium isotope probing;    carbon-deuterium ratio;    antimicrobial susceptibility test;    minimum inhibitory concentration;   

DOI  :  10.3389/fmicb.2022.876925

来源: DOAJ

【 摘 要 】

Nosocomial infection by multi-drug resistance Elizabethkingia spp. is an emerging concern with severe clinical consequences, particularly in immunocompromised individuals and infants. Efficient control of this infection requires quick and reliable methods to determine the appropriate drugs for treatment. In this study, a total of 31 Elizabethkingia spp., including two standard strains (ATCC 13253 and FMS-007) and 29 clinical isolates obtained from hospitals in China were subjected to single cell Raman spectroscopy analysis coupled with deuterium probing (single cell Raman-DIP). The results demonstrated that single cell Raman-DIP could determine antimicrobial susceptibility of Elizabethkingia spp. in 4 h, only one third of the time required by standard broth microdilution method. The method could be integrated into current clinical protocol for sepsis and halve the report time. The study also confirmed that minocycline and levofloxacin are the first-line antimicrobials for Elizabethkingia spp. infection.

【 授权许可】

Unknown