Efficacy of Peptone Glycerol Broth in Long-term Storage of the Bacterial and Yeast Cultures

Journal of Clinical and Diagnostic Research

卷:15

SR Manjula¹  A Gomathi Chitra¹  J Lavanya²  Venkata Chaithanya³  Ramya Sivaramakrishnan³ 

[1] Assistant Professor, Department of Microbiology, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.;

[2] Associate Professor, Department of Microbiology, KMCH Institute of Health Science and Research, Coimbatore, Tamil Nadu, India.;

[3] Postgraduate Student, Department of Microbiology, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.;

关键词: brucella glycerol broth;    cryobead;    cryopreservation technique;    skim milk;   

DOI  :  10.7860/JCDR/2021/47796.14524

来源: DOAJ

【 摘 要 】

Introduction: Bacterial isolates and control strains stockingis an integral part of clinical microbiology laboratories. This isan essential step in maintaining quality. Preserving the strainswithout altering the character is an essentiality. There aredifferent stock culture preparations studied in past showingvaried level of performance.Aim: To evaluate the performance in terms of longevity andphenotypic character preservation of Peptone Glycerol Broth(PGB) in comparison to Brucella Glycerol Broth (BGB) and SkimMilk (SKM).Materials and Methods: The present study was a prospectiveanalytical study. Three quality control strains and seven clinicalisolates with different types of resistance were stocked intriplicates with cryobead based peptone broth with 15% glycerol,Brucella Broth (BB) with 15% glycerol and 10% SKM and storedat -80°C. Isolates were revived in monthly pattern, quarterlypattern and once after 10 months to assess the variations inviability and loss of phenotypic properties arising out of repeatedfreeze thaw and contaminations. Viability was assessed bytime taken to produce observable confluent growth on revival.Metabolic characters and antibiotic susceptibility testing werecompared before and after stock revival at intervals.Results: The phenotypic characters like metabolic featuresand antibiotic susceptibility were preserved with all threepreparations both with repeated freeze thaw and single freezethaw at the end of 10 months. PGB and BGB had a 100%revival rate of stored isolates with a confluent growth at24 hours in comparison to 93.56% with SKM.Conclusion: Cryobead preparation of peptone broth-15%glycerol can be used as an effective preparation for stockculture maintenance of non-fastidious bacteria and yeast.

【 授权许可】

Unknown