A Comparison of the Molecular Organization of Genomic Regions Associated with Resistance to Common Bacterial Blight in Two Phaseolus vulgaris Genotypes

Frontiers in Plant Science

卷:4

Weilong eXie¹  Alireza eNavabi¹  Chun eShi²  Kangfu eYu²  K. Peter ePauls³  Gregory E. Perry³  Yarmilla eReinprecht³  Claudia eDiNatale⁴  William C Crosby⁴ 

[1] Agriculture and Agri-Food Canada;

[2] Agriculture and Agrifood Canada;

[3] University of Guelph;

[4] University of Windsor;

关键词: Disease Resistance;    Comparative genomics;    next generation sequencing;    Phaseolus vulgaris;    Common Bacterial Blight;   

DOI  :  10.3389/fpls.2013.00318

来源: DOAJ

【 摘 要 】

Resistance to common bacterial blight, caused by Xanthomonas axonopodis pv. phaseoli, in Phaseolus vulgaris is conditioned by several loci on different chromosomes. Previous studies with OAC-Rex, a CBB-resistant, white bean variety of Mesoamerican origin, identified two resistance loci associated with the molecular markers Pv-CTT001 and SU91, on chromosome 4 and 8, respectively. Resistance to CBB is assumed to be derived from an interspecific cross with Phaseolus acutifolius in the pedigree of OAC-Rex. Our current whole genome sequencing effort with OAC-Rex provided the opportunity to compare its genome in the regions associated with CBB resistance with the v1.0 release of the P. vulgaris line G19833, which is a large seeded bean of Andean origin, and (assumed to be) CBB susceptible.. In addition, the genomic regions containing SAP6, a marker associated with P. vulgaris-derived CBB-resistance on chromosome 10, were compared. These analyses indicated that gene content was highly conserved between G19833 and OAC-Rex across the regions examined (>80%). However, fifty-nine genes unique to OAC Rex were identified, with resistance gene homologues making up the largest category (10 genes identified). Two unique genes in OAC-Rex located within the SU91 resistance QTL have homology to P. acutifolius ESTs and may be potential sources of CBB resistance. As the genomic sequence assembly of OAC-Rex is completed, we expect that further comparisons between it and the G19833 genome will lead to a greater understanding of CBB resistance in bean.

【 授权许可】

Unknown