Toward a Global Public Repository of Community Protocols to Encourage Best Practices in Biomolecular Ocean Observing and Research

Frontiers in Marine Science

卷:8

Maxime Sweetlove¹  Susanna Theroux²  Jodie van de Kamp³  Alison Watts⁴  Raissa Meyer⁵  Christopher Meyer⁶  Nicholas W. Jeffery⁷  Neil Davies⁸  Christina Pavloudi⁹  Pier Luigi Buttigieg¹⁰  Kathleen Johnson Pitz¹¹  Robyn M. Samuel¹² 

[1] 0Royal Belgian Institute of Natural Sciences, Brussels, Belgium;

[2] 1Southern California Coastal Water Research Project, Costa Mesa, CA, United States;

[3] 2Commonwealth Scientific and Industrial Research Organization, Oceans and Atmosphere, Hobart, TAS, Australia;

[4] 3Civil and Environmental Engineering, University of New Hampshire, Durham, NH, United States;

[5] Alfred Wegener Institute Helmholtz Center for Polar and Marine Research, Bremerhaven, Germany;

[6] Department of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, DC, United States;

[7] Fisheries and Oceans Canada, Bedford Institute of Oceanography, Dartmouth, NS, Canada;

[8] Gump South Pacific Research Station, University of California, Berkeley, Moorea, French Polynesia;

[9] Hellenic Centre for Marine Research, Institute of Marine Biology, Biotechnology and Aquaculture, Heraklion, Greece;

[10] Helmholtz Metadata Collaboration, GEOMAR, Kiel, Germany;

[11] Monterey Bay Aquarium Research Institute, Moss Landing, CA, United States;

[12] Ocean Technology and Engineering Group, National Oceanography Center, Southampton, United Kingdom;

[13] School of Ocean and Earth Science, University of Southampton, Southampton, United Kingdom;

关键词: omics;    eDNA;    ocean best practices;    ocean observations;    metadata;    protocol management;   

DOI  :  10.3389/fmars.2021.758694

来源: DOAJ

【 摘 要 】

Biomolecular ocean observing and research is a rapidly evolving field that uses omics approaches to describe biodiversity at its foundational level, giving insight into the structure and function of marine ecosystems over time and space. It is an especially effective approach for investigating the marine microbiome. To mature marine microbiome research and operations within a global ocean biomolecular observing network (OBON) for the UN Decade of Ocean Science for Sustainable Development and beyond, research groups will need a system to effectively share, discover, and compare “omic” practices and protocols. While numerous informatic tools and standards exist, there is currently no global, publicly-supported platform specifically designed for sharing marine omics [or any omics] protocols across the entire value-chain from initiating a study to the publication and use of its results. Toward that goal, we propose the development of the Minimum Information for an Omic Protocol (MIOP), a community-developed guide of curated, standardized metadata tags and categories that will orient protocols in the value-chain for the facilitated, structured, and user-driven discovery of suitable protocol suites on the Ocean Best Practices System. Users can annotate their protocols with these tags, or use them as search criteria to find appropriate protocols. Implementing such a curated repository is an essential step toward establishing best practices. Sharing protocols and encouraging comparisons through this repository will be the first steps toward designing a decision tree to guide users to community endorsed best practices.

【 授权许可】

Unknown