CuO and CeO2 Nanostructures Green Synthesized Using Olive Leaf Extract Inhibits the Growth of Highly Virulent Multidrug Resistant Bacteria

Frontiers in Pharmacology

卷:9

Nyla Jabeen¹  Muhammad T. Pervez²  Gregory Franklin⁴  Ayesha Maqbool⁵  Mudassar Nazar⁶  Talib Hussain⁶  Qaisar Maqbool⁶ 

[1] Applied Biotechnology and Genetic Engineering Lab, Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Islamabad, Pakistan;

[2] Department of Bioinformatics and Computational Biology, Virtual University of Pakistan, Lahore, Pakistan;

[3] Department of Biotechnology, Virtual University of Pakistan, Lahore, Pakistan;

[4] Department of Integrative Plant Biology, Institute of Plant Genetics of the Polish Academy of Sciences, Poznan, Poland;

[5] Department of Molecular Biology, Virtual University of Pakistan, Lahore, Pakistan;

[6] National Institute of Vacuum Science and Technology, Islamabad, Pakistan;

关键词: green synthesis;    CuO-nanostructures;    CeO2-nanostructures;    multidrug-resistant bacteria;    cytotoxicity;    biocompatibility;   

DOI  :  10.3389/fphar.2018.00987

来源: DOAJ

【 摘 要 】

One of the major challenges of nano-biotechnology is to engineer potent antimicrobial nanostructures (NS) with high biocompatibility. Keeping this in view, we have performed aqueous olive leaf extract mediated one pot facile synthesis of CuO-NS and CeO2-NS. Prepared NS were homogenous, less than 26 nm in size, and small crystallite units as revealed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) analyses. Fourier transform infrared spectroscopy (FTIR) of CuO-NS and CeO2-NS showed typical Cu-O prints around 592–660 cm-1 and Ce-O bond vibrations at 453 cm-1. The successful capping of CuO-NS and CeO2-NS by compounds present in the plant extract was further validated by high performance liquid chromatography (HPLC) and thermal gravimetric analysis (TGA). Active phyto-chemicals from the leaf extract simultaneously acted as strong reducing as well as capping agent in the NS synthesis. NS engineered in the present study showed antibacterial potential at extremely low concentration against highly virulent multidrug-resistant (MDR) gram-negative strains (Escherichia coli, Enterobacter cloacae, Acinetobacter baumannii and Pseudomonas aeruginosa), alarmed by World Health Organization (WHO). Furthermore, CuO-NS and CeO2-NS did not show any cytotoxicity on HEK-293 cell lines and Brine shrimp larvae indicating that the NS green synthesized in the present study are biocompatible.

【 授权许可】

Unknown