Retrospective Genotyping and Whole Genome Sequencing of a Canine Parvovirus Outbreak in Bangladesh

Pathogens

卷:10

Saroj Kumar Yadav¹  Tofazzal Md Rakib²  Babu Kanti Nath³  Shane R. Raidal³  Shubhagata Das³  Tridip Das³ 

[1] Department of Medicine and Surgery, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh;

[2] Department of Pathology and Parasitology, Chattogram Veterinary and Animal Sciences University, Chattogram 4225, Bangladesh;

[3] School of Agriculture, Environment and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2650, Australia;

关键词: canine parvovirus;    whole genome shotgun sequencing;    PCR-HRM;    new CPV-2a;   

DOI  :  10.3390/pathogens10111373

来源: DOAJ

【 摘 要 】

Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV-2a as the contributing antigenic variant. Further genotyping using polymerase chain reaction coupled with high-resolution melt assays (PCR-HRM) confirmed new CPV-2a infection in all deceased dogs. PCR-HRM of additional thirty-four clinically suspected dogs suggested that this variant is in wider community circulation, at least in the southeastern part of Bangladesh. We present complete genome sequence of the new CPV-2a variant circulating in the domestic canine population of Bangladesh.

【 授权许可】

Unknown