Evaluation of DNA Damage in Type 1 Diabetes Patients

Journal of Clinical and Diagnostic Research

卷:13

HIVI MOHAMMED MAHMOUD¹  DHIA J ALTIMIMI² 

[1] Assistant Lecturer, Department of Medical Chemistry, College of Medicine, University of Duhok, Duhok, Kurdistan Region, Iraq.;

[2] Professor and Head, Department of Medical Chemistry, College of Medicine, University of Duhok, Duhok, Kurdistan Region, Iraq.;

关键词: 8-ohdg;    autoantibodies;    diabetes mellitus;    oxidative stress;   

DOI  :  10.7860/JCDR/2019/39851.12823

来源: DOAJ

【 摘 要 】

Introduction: DNA damage due to oxidative stress leads to progression of diabetic complications. The association between DNA damage and diabetes mellitus has prompted to study the extent of DNA damage and factors affecting this health problem in Type 1 Diabetes (T1D). Aim: To assess serum 8-hydroxy 2’deoxyguanosine (8-OHdG) levels as a DNA damage biomarker in T1D patients. Materials and Methods: The study was carried out between September 2017 and March 2018 at Duhok Diabetes Center, Duhok, Kurdistan Region (Iraq). Serum 8-OHdG levels of 132 T1D patients and 123 age and sex matched healthy control subjects were estimated using ELISA technique. The Insulin Autoantibodies (IAA), Islets Cell Antibodies (ICA) and Glutamic Acid Decarboxylase Antibodies (GADA) were assessed in T1D patients. Results: The results revealed that serum 8-OHdG levels were significantly raised in patients (6.02±2.05 ng/mL) as compared to healthy controls (2.03±1.63 ng/mL) (p<0.01). Mean 8-OHdG was also found to be significantly higher in patients with autoantibodies (6.20±2.12 ng/mL) as compared to patients without autoantibodies (5.7±1.93 ng/mL) (p=0.04). Moreover, the prevalence of DNA damage was found to be significantly higher in patients with autoantibodies (79.3%) as compared to patients without autoantibodies (40.0%) (p=0.02). Conclusion: The present study has indicated that DNA damage accompanied by autoantibodies is highly exhibited in patients with T1D. Antioxidant supplementation may be an effective public heath intervention to reduce DNA damage and oxidative stress.

【 授权许可】

Unknown