Analysis of insoluble proteins

BioTechniques

卷:46

Sarah Trimpin¹  Bill Brizzard² 

[1] 1Department of Chemistry, Wayne State University, Department of Chemistry, Detroit, Michigan, USA;

[2] 2Indiana University Research and Technology Corporation, Bloomington, Indiana, USA;

关键词: fusion tag;    recombinant protein;    solvent-free mass spectrometry;    MALDI;    IMS;   

DOI  :  10.2144/000113135

来源: DOAJ

【 摘 要 】

The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20–30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time consuming. This review presents an overview of methods for purification of expressed proteins using fusion tags as well as methods for analysis of insoluble proteins by mass spectrometry with a goal of achieving high-throughput analysis.

【 授权许可】

Unknown