Identification of Tilletia species using rep-PCR fingerprinting technique

Genetika

卷:43

关键词: identification;    rep-PCR fingerprinting;    Tilletia tritici;    Tilletia controversa;    Tilletia laevis;    Tilletia bromi;    Tilletia walkeri;    wheat;   

DOI  :  10.2298/GENSR1101183Z

来源: DOAJ

【 摘 要 】

Analyzing 167 non-processed seed samples of wheat, it was found that 145samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %)samples were not contaminated. By using rep-PCR fingerprinting technique, itwas found that DNA isolates of T. tritici originated from Serbian wheatsamples had 80 % similarity with positive control for T. tritici. One isolateshared similarity of 60% with T. tritici, T. controversa and T. laevis. Itwas supposed that this isolate belongs to T. bromi. Isolate of T. laevisshared a similarity of 70 % with isolates of T. tritici and T. controversa,while T. walkeri was more than 10 % similar with T. tritici, T. controversaand T. laevis. Although T. controversa and T. tritici had high percent ofgenetic similarity, they were clustered separately. Our results suggest thatrep-PCR fingerprinting could be a useful tool for monitoring presence ofmorphologically similar Tilletia species in wheat production areas.

【 授权许可】

Unknown