Genetika | 卷:43 |
Identification of Tilletia species using rep-PCR fingerprinting technique | |
关键词: identification; rep-PCR fingerprinting; Tilletia tritici; Tilletia controversa; Tilletia laevis; Tilletia bromi; Tilletia walkeri; wheat; | |
DOI : 10.2298/GENSR1101183Z | |
来源: DOAJ |
【 摘 要 】
Analyzing 167 non-processed seed samples of wheat, it was found that 145samples (86.8 %) were contaminated with Tilletia species, while 22 (13.2 %)samples were not contaminated. By using rep-PCR fingerprinting technique, itwas found that DNA isolates of T. tritici originated from Serbian wheatsamples had 80 % similarity with positive control for T. tritici. One isolateshared similarity of 60% with T. tritici, T. controversa and T. laevis. Itwas supposed that this isolate belongs to T. bromi. Isolate of T. laevisshared a similarity of 70 % with isolates of T. tritici and T. controversa,while T. walkeri was more than 10 % similar with T. tritici, T. controversaand T. laevis. Although T. controversa and T. tritici had high percent ofgenetic similarity, they were clustered separately. Our results suggest thatrep-PCR fingerprinting could be a useful tool for monitoring presence ofmorphologically similar Tilletia species in wheat production areas.
【 授权许可】
Unknown