Combined Use of Vinasse and Nitrogen as Fertilizers Affects Nitrification, Ammonification, and Denitrification by Prokaryotes

Frontiers in Soil Science

卷:1

Miriam Gonçalves de Chaves¹  Siu Mui Tsai¹  Eiko Eurya Kuramae³  Dayane Juliate Barros⁴  Acacio Aparecido Navarrete⁵  Luis Fernando Merloti⁶  Andressa Monteiro Venturini⁷  Raffaella Rossetto⁸ 

[1] Cell and Molecular Biology Laboratory, Center for Nuclear Energy in Agriculture, University of São Paulo (USP), Piracicaba, Brazil;

[2] Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Netherlands;

[3] Ecology and Biodiversity Group, Utrecht University, Utrecht, Netherlands;

[4] Graduate Program in Biodiversity and Biotechnology, Federal University of Tocantins, Palmas, Brazil;

[5] Graduate Programa in Environmental Sciences, University Brazil, Fernandópolis, Brazil;

[6] Luiz de Queiroz College of Agriculture, University of São Paulo (USP), Piracicaba, Brazil;

[7] Princeton Institute for International and Regional Studies (PIIRS), Princeton University, Princeton, NJ, United States;

[8] São Paulo's Agency for Agribusiness Technology, Piracicaba, Brazil;

关键词: Anaeromyxobacter;    nitrogen cycling;    sugarcane soil;    Natronomonas;    p450nor;   

DOI  :  10.3389/fsoil.2021.746745

来源: DOAJ

【 摘 要 】

A common agricultural practice of combining organic fertilizer vinasse (a liquid residue from sugarcane ethanol production) with mineral nitrogen (N) fertilizer promotes N losses such as greenhouse gas emissions due to the effects of physicochemical changes in soil on the microbiota inhabiting this environment. In this study, we applied microarray GeoChip v.5.0M technology to obtain a better insight into the prokaryotic communities and identify and quantify the N functional gene families associated with the N processes in sugarcane soils without N fertilizer (N0), with urea at 60 kg ha−1 (N60), and with vinasse combined with urea (NV). Soil samples were collected at 7 (T7) and 150 (T150) days after N application, corresponding to maximum and minimum nitrous oxide (N2O) emissions, respectively, for molecular and physicochemical analysis. Additionally, the metagenomes of these DNA samples, previously deposited in the MG-RAST server, were accessed to investigate the functions and taxonomic groups associated with selected gene families. The results revealed that 87% of the select gene families were significantly responsive to the fertilizer combined treatment (NV) in the 7 days after the application. The most responsive genes and processes were nitrification [with the amoA gene from ammonia-oxidizing Bacteria (AOB) and Archaea (AOA) and hao from Bacteria], ammonification (with gdh and ureC genes from Bacteria and Archaea), and denitrification (with p450nor from Eukarya). The AOA, Nitrosopumilus, and AOB, Nitrosomonas, were the groups with the greatest functions associated with nitrification, as well as a pathogenic Mycobacterium, with denitrification. The results also revealed that under N fertilizers and decreased O2 in soil, the increases in K and P nutrients can promote the growth of the halophile Archaea Natronomonas and the Bacteria Anaeromyxobacter, which can reduce N2O. In conclusion, this typical agricultural fertilization management may favor functional genes and archaeal and bacterial groups associated with N processes that have the potential to reduce environmental damage in tropical sugarcane soils.

【 授权许可】

Unknown