Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases

Journal of Translational Medicine

卷:15

Thomas Pickardt¹  Andreas Keller²  Nicole Ludwig³  Eckart Meese³  Masood Abu-Halima³  Janine Mark⁴  Martin Poryo⁴  Hashim Abdul-Khaliq⁴  Ina Marsollek⁴  Johannes Petersen⁴  Hans-Joachim Schäfers⁵  Christian Giebels⁵  Ulrich Grundmann⁶ 

[1] Competence Network for Congenital Heart Defects, National Register for Congenital Heart Defects, DZHK;

[2] Department of Clinical Bioinformatics, Saarland University;

[3] Department of Human Genetics, Saarland University;

[4] Department of Pediatric Cardiology, Saarland University Medical Center;

[5] Department of Thoracic and Cardiovascular Surgery, Saarland University Medical Center;

[6] Department of Anaesthesiology, Intensive Care and Pain Therapy, Saarland University Medical Center;

关键词: MicroRNA;    Congenital heart disease;    Atrial myocardium;    Cardiopulmonary bypass;   

DOI  :  10.1186/s12967-017-1213-9

来源: DOAJ

【 摘 要 】

Abstract Background Children with congenital heart defects (CHDs) are at high risk for myocardial failure after operative procedures with cardiopulmonary bypass (CPB). Recent studies suggest that microRNAs (miRNA) are involved in the development of CHDs and myocardial failure. Therefore, the aim of this study was to determine alterations in the miRNA profile in heart tissue after cardiac surgery using CPB. Methods In total, 14 tissue samples from right atrium were collected from patients before and after connection of the CPB. SurePrint™ 8 × 60K Human v21 miRNA array and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were employed to determine the miRNA expression profile from three patients before and after connection of the CPB. Enrichment analyses of altered miRNA expression were predicted using bioinformatic tools. Results According to miRNA array, a total of 90 miRNAs were significantly altered including 29 miRNAs with increased and 61 miRNAs with decreased expression after de-connection of CPB (n = 3) compared to before CPB (n = 3). Seven miRNAs had been validated using RT-qPCR in an independent cohort of 11 patients. Enrichment analyses applying the KEGG database displayed the highest correlation for signaling pathways, cellular community, cardiovascular disease and circulatory system. Conclusion Our result identified the overall changes of the miRNome in right atrium tissue of patients with CHDs after CPB. The differentially altered miRNAs lay a good foundation for further understanding of the molecular function of changed miRNAs in regulating CHDs and after CPB in particular.

【 授权许可】

Unknown