Protocol for the measurement of fatty acid and glycerol turnover in vivo in baboons[S]

Journal of Lipid Research

卷:52

Vidya Vaidyanathan¹  Sonya M. Veron¹  Maggie Garcia-Forey²  Raul A. Bastarrachea²  V. Saroja Voruganti²  Paul B. Higgins²  Elizabeth J. Parks³ 

[1] Center for Human Nutrition, University of Texas Southwestern Medical Center, Dallas, TX;

[2] Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX;

[3] To whom correspondence should be addressed. [e-mail];

关键词: adipose tissue;    fatty acid metabolism;    glycolipids;    lipids;    lipolysis;    lipoprotein kinetics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Recognition of the strength of nonhuman primate models in investigating metabolic disorders has resulted in an expanded need for in vivo research techniques. We studied adipose metabolism in 10 baboons (13.0 ± 4.2 years old, 29.5 ± 5.5 kg). Part 1 evaluated the effect of different sedatives on the rate of appearance of plasma free fatty acids (RaFFA), assessed using 13C4-labeled palmitate infusion (7 µmol/kg/min). Animals, were studied with no sedation, with complete isoflurane sedation, and with minimal midazolam infusion (0.04 mg/kg/h), with the last scheme allowing for the most consistent values and animals that were visually more calm. In Part 2, RaFFA and RaGlycerol (D5-glycerol, 5 mg/kg lean body mass/h) were measured. From midnight to 0300, flux fell and came to a steady state between 0500 and 0700 h (RaFFA, 39.4 ± 29.8 μmol/kg fat mass/min; and RaGlycerol, 26.9 ± 7.3 μmol/kg/min). The RaFFA-to-RaGlycerol ratio was 1.5 ± 0.8 (49% reesterification). The decline in turnover throughout the night reflects natural circadian processes and was mirrored by reductions in FFA and glycerol to 0.62 and ± 0.14 and 0.16 and ± 0.03 mmol/l, respectively. The concurrent changes in both FFA and glycerol kinetics indicate physiologic validity of the method. These techniques will support needed research to determine mechanisms by which treatments act upon the adipocyte in vivo.

【 授权许可】

Unknown