Dopamine Modulates Homeostatic Excitatory Synaptic Plasticity of Immature Dentate Granule Cells in Entorhino-Hippocampal Slice Cultures

Frontiers in Molecular Neuroscience

卷:11

Christos Galanis¹  Andreas Vlachos¹  Andreas Strehl²  Tijana Radic²  Thomas Deller²  Stephan Wolfgang Schwarzacher² 

[1] Department of Neuroanatomy, Institute of Anatomy and Cell Biology, Faculty of Medicine, University of Freiburg, Freiburg, Germany;

[2] Neuroscience Center, Institute of Clinical Neuroanatomy, Goethe-University Frankfurt, Frankfurt, Germany;

关键词: homeostasis;    synaptic scaling;    neurogenesis;    hippocampus;    neuromodulation;    stem cells;   

DOI  :  10.3389/fnmol.2018.00303

来源: DOAJ

【 摘 要 】

Homeostatic plasticity mechanisms maintain neurons in a stable state. To what extent these mechanisms are relevant during the structural and functional maturation of neural tissue is poorly understood. To reveal developmental changes of a major homeostatic plasticity mechanism, i.e., homeostatic excitatory synaptic plasticity, we analyzed 1-week- and 4-week-old entorhino-hippocampal slice cultures and investigated the ability of immature and mature dentate granule cells (GCs) to express this form of plasticity. Our experiments demonstrate that immature GCs are capable of adjusting their excitatory synaptic strength in a compensatory manner at early postnatal stages, i.e., in 1-week-old preparations, as is the case for mature GCs. This ability of immature dentate GCs is absent in 4-week-old slice cultures. Further investigations into the signaling pathways reveal an important role of dopamine (DA), which prevents homeostatic synaptic up-scaling of immature GCs in young cultures, whereas it does not affect immature and mature GCs in 4-week-old preparations. Together, these results disclose the ability of immature GCs to express homeostatic synaptic plasticity during early postnatal development. They hint toward a novel role of dopaminergic signaling, which may gate activity-dependent changes of newly born neurons by blocking homeostasis.

【 授权许可】

Unknown