miRNA-99b-5p targets FZD8 to inhibit non-small cell lung cancer proliferation, migration and invasion

OncoTargets and Therapy

卷:Volume 12

关键词: miR-99b-5p;    FZD8;    non-small cell lung cancer;    migration;    invasion;   

DOI  :  

来源: DOAJ

【 摘 要 】

Rui Liu,1,2 Yajuan Chen,1 Tao Shou,2 Jing Hu,2 Chen Qing11Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650031, People’s Republic of China; 2Department of Oncology, The First People’s Hospital of Yunnan Province, Kunming 650032, Yunnan, People’s Republic of ChinaBackground: miRNAs were found to play crucial roles in regulating cellular behaviors. The aim of this study was to investigate the biological function of miRNA-99b-5p (miR-99b-5p) in non-small cell lung cancer (NSCLC).Materials and methods: miR-99b-5p expression level in NSCLC cell lines was detected by quantitative real-time PCR (qRT-PCR). Cell proliferation, migration and invasion were examined by cell counting kit-8 (CCK-8) assay, wound-healing assay and Transwell invasion assay, respectively. Dual-luciferase activity reporter assay and Western blot assay were conducted to validate the target of miR-99b-5p.Results: The expression of miR-99b-5p was decreased in NSCLC cell lines compared with normal cell line. Overexpression of miR-99b-5p inhibits cell proliferation, migration and invasion in vitro. FZD8 was validated as a direct target of miR-99b-5p. Overexpression of FZD8 partially abolished the effects of miR-99b-5p mimic on NSCLC cell behaviors.Conclusion: Collectively, our results demonstrated that miR-99b-5p inhibits NSCLC cell proliferation, migration and invasion through targeting FZD8. This newly identified miR-99b-5p/FZD8 axis provided novel insights into the mechanisms underlying NSCLC progression.Keywords: miR-99b-5p, FZD8, non-small cell lung cancer, migration, invasion

【 授权许可】

Unknown