Curcumin Mitigates Immune-Induced Epithelial Barrier Dysfunction by Campylobacter jejuni

International Journal of Molecular Sciences

卷:20

Kerstin Stingl¹  JuliaC. Golz¹  Corinna Kehrenberg²  Roland Bücker³  PraveenKumar Nattramilarasu³  Anja Fromm³  Eduard Butkevych³  FábiaDaniela Lobo de Sá³  Jörg-Dieter Schulzke³  Stefan Bereswill⁴  Soraya Mousavi⁴  MarkusM. Heimesaat⁴  Verena Moos⁵  Diana Seinige⁶  Sophie Kittler⁶ 

[1] German Federal Institute for Risk Assessment (BfR), Department of Biological Safety, National Reference Laboratory for Campylobacter, 12277 Berlin, Germany;

[2] Institute for Veterinary Food Science, Justus-Liebig-University, 35392 Giessen, Germany;

[3] Institute of Clinical Physiology/Nutritional Medicine, Medical Department, Division of Gastroenterology, Infectiology, Rheumatology, Charité–Universitätsmedizin Berlin, 12203 Berlin, Germany;

[4] Institute of Microbiology, Infectious Diseases and Immunology, Charité–Universitätsmedizin Berlin, Campus Benjamin Franklin, 14195 Berlin, Germany;

[5] Medical Department, Division of Gastroenterology, Infectiology and Rheumatology, Charité–Universitätsmedizin Berlin, 12203 Berlin, Germany;

[6] University of Veterinary Medicine Hannover, Research Center for Emerging Infections and Zoonoses, 30559 Hannover, Germany;

关键词: campylobacter jejuni;    curcumin;    tight junction;    claudin;    apoptosis;    co-culture;    mouse colon;    cytokines;    tnf;    nfκb;   

DOI  :  10.3390/ijms20194830

来源: DOAJ

【 摘 要 】

Campylobacter jejuni (C. jejuni) is the most common cause of foodborne gastroenteritis worldwide. The bacteria induce diarrhea and inflammation by invading the intestinal epithelium. Curcumin is a natural polyphenol from turmeric rhizome of Curcuma longa, a medical plant, and is commonly used in curry powder. The aim of this study was the investigation of the protective effects of curcumin against immune-induced epithelial barrier dysfunction in C. jejuni infection. The indirect C. jejuni-induced barrier defects and its protection by curcumin were analyzed in co-cultures with HT-29/B6-GR/MR epithelial cells together with differentiated THP-1 immune cells. Electrophysiological measurements revealed a reduction in transepithelial electrical resistance (TER) in infected co-cultures. An increase in fluorescein (332 Da) permeability in co-cultures as well as in the germ-free IL-10^−/− mouse model after C. jejuni infection was shown. Curcumin treatment attenuated the C. jejuni-induced increase in fluorescein permeability in both models. Moreover, apoptosis induction, tight junction redistribution, and an increased inflammatory response—represented by TNF-α, IL-1β, and IL-6 secretion—was observed in co-cultures after infection and reversed by curcumin. In conclusion, curcumin protects against indirect C. jejuni-triggered immune-induced barrier defects and might be a therapeutic and protective agent in patients.

【 授权许可】

Unknown