Evaluation of Antioxidant Activity of Spice-Derived Phytochemicals Using Zebrafish

International Journal of Molecular Sciences

卷:21

Makoto Kobayashi¹  Yuka Endo¹  Kyoji Muraki¹  Yuji Fuse¹ 

[1] Department of Molecular and Developmental Biology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan;

关键词: antioxidant activity;    food ingredient;    h₂o₂;    6-msitc;    naaso₂;    nrf2;    oxidative stress;    dietary phytochemical;    spice;    zebrafish;   

DOI  :  10.3390/ijms21031109

来源: DOAJ

【 摘 要 】

Various dietary phytochemicals seem to display antioxidant activity through the NF-E2-related factor 2 (Nrf2) pathway. However, few studies have demonstrated its antioxidant effect and Nrf2 dependency at the animal level. We constructed a zebrafish-based assay system to analyze the in vivo antioxidant activity of phytochemicals and examined the activity of 10 phytochemicals derived from spices, using this system as a pilot study. Hydrogen peroxide and arsenite were used as oxidative stressors, and Nrf2 dependency was genetically analyzed using an Nrf2-mutant zebrafish line. The activities of curcumin, diallyl trisulfide and quercetin were involved in the reduction of hydrogen peroxide toxicity, while those of cinnamaldehyde, isoeugenol and 6-(methylsulfinyl)hexyl isothiocyanate were involved in the reduction of arsenite toxicity. The antioxidant activities of these phytochemicals were all Nrf2 dependent, with the exception of cinnamaldehyde, which showed strong antioxidant effects even in Nrf2-mutant zebrafish. In summary, we succeeded in constructing an assay system to evaluate the in vivo antioxidant activity of various phytochemicals using zebrafish larvae. Using this system, we found that each spice-derived phytochemical has its own specific property and mechanism of antioxidant action.

【 授权许可】

Unknown