Method for organotypic tissue culture in the aged animal

MethodsX

卷:4

Gurdeep Marwarha¹  Jared Schommer¹  Othman Ghribi¹  Matthew Schrag²  Alexander Nackenoff² 

[1] Biomedical Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, United States;

[2] Department of Neurology, Vanderbilt University School of Medicine, Nashville, TN, United States;

关键词: We are describing a method for organotypic tissue culture in the aged animal in this manuscript;   

DOI  :  10.1016/j.mex.2017.03.003

来源: DOAJ

【 摘 要 】

Organotypic slicing of brain tissue from young rodents has been used as a powerful model system for biomedical research [1–3]. Organotypic slicing complements cell culture and in vivo studies in multiple facets. This system can be useful for investigating manipulation of cellular signaling pathways without the hindrance of the blood-brain barrier while sacrificing fewer animals in the process. It also allows for preserved cellular connectivity and local intact circuitry which is a drawback of isolated cell cultures. Studies on age-related diseases have mainly used embryonic or early postnatal organotypic slice tissue. Excluding synaptic plasticity studies that are usually carried-out over a few hours and use adult mice or rats, a handful of studies performed on adult animals have had success for survival of slices [4,5]. Here we describe a method for culturing organotypic slices with high viability from hippocampus of aged mice and rabbits.• Our method permits slices from mice as old as 16 months and rabbits as old as years of age to survive ex vivo up to 8 weeks [6–9]. Such a slice system may be relevant to investigating age-related brain diseases.

【 授权许可】

Unknown