Intranasal HSP70 administration protects against dopaminergic denervation and modulates neuroinflammatory response in the 6-OHDA rat model

Brain, Behavior, & Immunity - Health

卷:14

Daniel Oppermann Peixoto¹  Pedro Ozorio Brum²  Juciano Gasparotto²  Daniel Pens Gelain²  Carolina Saibro-Girardi²  Reykla Ramon Bittencourt²  Alexandre Kleber Silveira²  Flávio Gabriel Carazza-Kessler²  Nauana Somensi²  Laíssa Santos Soares²  Luiza Marques Prates Behrens²  Lucas Santos²  José Cláudio Fonseca Moreira²  Jade de Oliveira²  Camila Tiefensee Ribeiro³ 

[1] Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;

[2] Corresponding author. Rua Ramiro Barcelos, 2600 – anexo, CEP 90035-003, Porto Alegre, RS, Brazil.;

关键词: Exogenous HSP70;    Intranasal treatment;    6-OHDA;    Neuroprotection;    Neuroinflammation;    Neurodegenerative diseases;   

DOI  :  

来源: DOAJ

【 摘 要 】

HSP70 is one of the main molecular chaperones involved in the cellular stress response. Besides its chaperone action, HSP70 also modulates the immune response. Increased susceptibility to toxic insults in intra- and extracellular environments has been associated with insufficient amounts of inducible HSP70 in adult neurons. On the other hand, exogenous HSP70 administration has demonstrated neuroprotective effects in experimental models of age-related disorders. In this regard, this study investigated the effects of exogenous HSP70 in an animal model of dopaminergic denervation of the nigrostriatal axis. After unilateral intrastriatal injection with 6-hydroxydopamine (6-OHDA), the animals received purified recombinant HSP70 through intranasal administration (2 μg/rat/day) for 15 days. Our results indicate a neuroprotective effect of intranasal HSP70 against dopaminergic denervation induced by 6-OHDA. Exogenous HSP70 improved motor impairment and reduced the loss of dopaminergic neurons caused by 6-OHDA. Moreover, HSP70 modulated neuroinflammatory response in the substantia nigra, an important event in Parkinson’s disease pathogenesis. Specifically, HSP70 treatment reduced microglial activation and astrogliosis induced by 6-OHDA, as well as IL-1β mRNA expression in this region. Also, recombinant HSP70 increased the protein content of HSP70 in the substantia nigra of rats that received 6-OHDA. These data suggest the neuroprotection of HSP70 against dopaminergic neurons damage after cellular stress. Finally, our results indicate that HSP70 neuroprotective action against 6-OHDA toxicity is related to inflammatory response modulation.

【 授权许可】

Unknown