Mass Spectrometry Reveals Molecular Structure of Polyhydroxyalkanoates Attained by Bioconversion of Oxidized Polypropylene Waste Fragments

Polymers

卷:11

Paweł Chaber¹  Wanda Sikorska¹  Magdalena Zięba¹  Marta Musioł¹  Grazyna Adamus¹  AdamA. Marek²  David Barsi³  VassilkaIvanova Ilieva³  Emo Chiellini³  AnabelItohowo Ekere⁴  Brian Johnston⁴  Barbara Mendrek⁴  Iza Radecka⁴  Marek Kowalczuk⁴ 

[1] Centre of Polymer and Carbon Materials, Polish Academy of Sciences, 41-800 Zabrze, Poland;

[2] Department of Chemical Organic Technology and Petrochemistry, Silesian University of Technology, 44-100 Gliwice, Poland;

[3] Laboratorio Materiali Polimerici Ecocompatibili (LMPE), via Nuova, 44/a, Segromigno in Monte, 55018 Capannori (LU), Italy;

[4] Wolverhampton School of Biology, Chemistry and Forensic Science, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton WV1 1LY, UK;

关键词: electrospray ionization dual mass spectrometry (esi-ms/ms);    polyhydroxyalkanoate (pha);    polypropylene (pp);    prodegraded;    cupriavidus necator;    coral reef;    bioplastics;    recycling;   

DOI  :  10.3390/polym11101580

来源: DOAJ

【 摘 要 】

This study investigated the molecular structure of the polyhydroxyalkanoate (PHA) produced via a microbiological shake flask experiment utilizing oxidized polypropylene (PP) waste as an additional carbon source. The bacterial strain Cupriavidus necator H16 was selected as it is non-pathogenic, genetically stable, robust, and one of the best known producers of PHA. Making use of PHA oligomers, formed by controlled moderate-temperature degradation induced by carboxylate moieties, by examination of both the parent and fragmentation ions, the ESI-MS/MS analysis revealed the 3-hydroxybutyrate and randomly distributed 3-hydroxyvalerate as well as 3-hydroxyhexanoate repeat units. Thus, the bioconversion of PP solid waste to a value-added product such as PHA tert-polymer was demonstrated.

【 授权许可】

Unknown