Biodegradation of Deoxynivalenol by Nocardioides sp. ZHH-013: 3-keto-Deoxynivalenol and 3-epi-Deoxynivalenol as Intermediate Products

Frontiers in Microbiology

卷:12

Fei Zheng¹  Xiangming Xie¹  Yao Bin²  Honghai Zhang²  Yuan Wang²  Xing Qin²  Heng Zhang²  Xiaolu Wang²  Huiying Luo² 

[1] College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China;

[2] Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China;

关键词: deoxynivalenol;    3-epi-DON;    3-keto-DON;    Nocardioides;    biodegradation;   

DOI  :  10.3389/fmicb.2021.658421

来源: DOAJ

【 摘 要 】

Deoxynivalenol (DON) is one of the most devastating and notorious contaminants in food and animal feed worldwide. A novel DON-degrading strain, Nocardioides sp. ZHH-013, which exhibited complete mineralization of DON, was isolated from soil samples. The intermediate products of DON generated by this strain were identified by high-performance liquid chromatography and ultra-performance liquid chromatography tandem mass spectrometry analyses. It was shown that, on an experimental level, 3-keto-DON was a necessary intermediate product during the conversion from DON to 3-epi-DON. Furthermore, the ZHH-013 strain could also utilize 3-epi-DON. This DON degradation pathway is a safety concern for food and feed. The mechanism of DON and 3-epi-DON elimination will be further studied, so that new enzymes for DON degradation can be identified.

【 授权许可】

Unknown