Effect of endophytic Bacillus and arbuscular mycorrhiza fungi (AMF) against Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici

Egyptian Journal of Biological Pest Control

Nongthombam Olivia Devi¹  Sushanti Thokchom¹  Monika Hajong¹  R. K. Tombisana Devi¹  Manashi Debbarma¹ 

[1] School of Crop Protection, College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, 793103, Umiam, Meghalaya, India;

关键词: Bacillus;    Arbuscular mycorrhizal fungi;    Meghalaya;    Isolates;    Field experiments;   

DOI  :  10.1186/s41938-021-00499-y

来源: Springer

【 摘 要 】

BackgroundFusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici (FOL) is a serious disease that causes significant economic losses in tomato production. Seventeen endophytic Bacillus isolates from tomato roots of Meghalaya were tested for antagonistic and plant growth promotion activities. Dominating arbuscular mycorrhiza fungi (AMF) spores were isolated from the rhizosphere soils of tomato grown in Meghalaya. The effect of different combinations of AMF and endophytic Bacillus on Fusarium wilt severity and growth of tomato plant under pot and field conditions was studied.ResultsThe endophytic Bacillus isolates ERBS51 and ERBS10 showed a maximum inhibition against FOL, with 58.43 and 55.68%, respectively, in a dual culture experiment. ERBS51 and ERBS10 were identified as Bacillus velezensis and Bacillus sp., respectively, based on 16s rRNA sequencing. Both isolates were found positive for iturin A, surfactin, bacillomycin D, protease, cellulase, pectinase, alpha-amylase, siderophore, ammonia production and ZnCO3 solubilization. Funneliformis mosseae and Glomus fasciculatum were the dominating AMF species in tomato rhizosphere of Meghalaya. The result of pot and field experiments revealed that out of all the treatments, combination of Funneliformis mosseae + Glomus fasciculatum + Bacillus velezensis + Bacillus sp. was shown to be the best in reducing the severity of Fusarium wilt to 77.44 and 66.74%, respectively. F. mosseae + G. fasciculatum + B. velezensis + Bacillus sp. also recorded the highest in most growth attributes and yield.ConclusionsEndophytic Bacillus (B. velezensis and Bacillus sp.) and AMF (F. mosseae and G. fasciculatum) were safe and effective biocontrol agents against Fusarium wilt of tomato.

【 授权许可】

CC BY