LncRNA MIR205HG accelerates cell proliferation, migration and invasion in hepatoblastoma through the activation of MAPK signaling pathway and PI3K/AKT signaling pathway

Biology Direct

Feng Liang¹  Fei Li¹  Qingfeng Li²  Jie Lei²  Zhibo Jiao²  Hong Sun²  Wei Zhang² 

[1] Department of Hepatobiliary Surgery, The First Affiliated Hospital of Hebei North University, 075000, Zhangjiakou, Hebei, China;Department of Pediatric Surgery, the First Affiliated Hospital of Hebei North University, No. 12 Changqing Road, Qiaoxi District, 075000, Zhangjiakou, Hebei, China;

关键词: Hepatoblastoma;    MIR205HG;    miR-514a-5p;    miR-205-5p;    MAPK9;   

DOI  :  10.1186/s13062-021-00309-3

来源: Springer

【 摘 要 】

BackgroundHepatoblastoma (HB) is identified to be the most common liver malignancy which occurs in children. Long non-coding RNAs (lncRNAs) have been implicated in numerous biological processes and diseases, including HB. LncRNA MIR205 host gene (MIR205HG) has been investigated in multiple cancers, however, its role in HB remains to be elucidated.MethodsMIR205HG expression was analyzed by RT-qPCR. EdU, colony formation and transwell assays were implemented to measure the biological function of MIR205HG on the progression of HB. Mechanism assays were carried out to probe into the underlying mechanism of MIR205HG in HB cells.ResultsMIR205HG was significantly overexpressed in HB. Moreover, MIR205HG inhibition suppressed the proliferative, migratory and invasive capacities of HB cells. Furthermore, MIR205HG competitively bound to microRNA-514a-5p (miR-514a-5p) and targeted mitogen-activated protein kinase 9 (MAPK9) to stimulate mitogen activated protein kinase (MAPK) signaling pathway. Besides, MIR205HG also served as a sponge for microRNA-205-5p (miR-205-5p) to activate the PI3K/AKT signaling pathway.ConclusionMIR205HG drives the progression of HB which might provide an efficient marker and new therapeutic target for HB.

【 授权许可】

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