N7-methylguanosine modification of lncRNAs in a rat model of hypoxic pulmonary hypertension: a comprehensive analysis

BMC Genomics

Ren Biao Chen¹  Rui Feng Zhang²  Huan Wang²  Si Ni Zhang² 

[1] Department of Radiology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310000, Hangzhou, People’s Republic of China;Department of Respiratory Medicine, Zhongda Hospital of Southeast University, 87 Dingjiaqiao Road, 210009, Nanjing, People’s Republic of China;

关键词: Hypoxic pulmonary hypertension;    lncRNAs;    N7-methylguanosine modification;    m7G-sequencing;   

DOI  :  10.1186/s12864-021-08188-8

来源: Springer

【 摘 要 】

BackgroundLong non-coding RNAs (lncRNAs) play a critical role in the pathogenesis of hypoxic pulmonary hypertension (HPH). The role of N7-methylguanosine (m7G) modification in lncRNAs has received increased attentions in recent years. However, the m7G-methylation of lncRNA in HPH has yet to be determined. We have therefore performed a transcriptome-wide analysis of m7G lncRNAs in HPH.ResultsDifferentially-expressed m7Gs were detected in HPH, and m7G lncRNAs were significantly upregulated compared with non-m7G lncRNAs in HPH. Importantly, this was the first time that the upregulated m7G lncXR_591973 and m7G lncXR_592398 were identified in HPH.ConclusionThis study provides the first m7G transcriptome-wide analysis of HPH. Importantly, two HPH-associated m7G lncRNAs were identified, although their clinical significance requires further validation.

【 授权许可】

CC BY