microRNA-155-3p delivered by M2 macrophages-derived exosomes enhances the progression of medulloblastoma through regulation of WDR82

Journal of Translational Medicine

Ruihe Shi¹  Qingrong Xu¹  Bin Luan¹  Xiufang Wang¹  Li Song¹ 

[1] Department of Pediatric Respiratory Medicine, The 3rd Affiliated Hospital of Zhengzhou University, No. 7, Kangfuqian street, 450052, Zhengzhou, Henan, China;

关键词: Medulloblastoma;    M2 macrophages;    Exosomes;    MicroRNA-155-3p;    WD repeat domain 82;    Proliferation;    Invasion;    Migration;    Apoptosis;   

DOI  :  10.1186/s12967-021-03156-y

来源: Springer

【 摘 要 】

ObjectiveExosomes, membranous nanovesicles, naturally bringing proteins, mRNAs, and microRNAs (miRNAs), play crucial roles in tumor pathogenesis. This study was to investigate the role of miR-155-3p from M2 macrophages-derived exosomes (M2-Exo) in promoting medulloblastoma (MB) progression by mediating WD repeat domain 82 (WDR82).MethodsmiR-155-3p expression was detected by RT-qPCR. The relationship of miR-155-3p with clinicopathological features of MB patients was analyzed. M2-Exo were isolated and identified by TEM, NTA and Western blot. CCK-8 assay, colony formation assay, flow cytometry, wound healing assay, and Transwell assay were performed to explore the role of miR-155-3p-enriched M2-Exo on the progression of MB cells. Luciferase assay were used to identify the relationship between miR-155-3p and WDR82. The effect of miR-155-3p-enriched M2-Exo on tumorigenesis of MB was confirmed by the xenograft nude mice model.ResultsmiR-155-3p was up-regulated in MB tissues of patients and MB cell lines. High miR-155-3p expression was correlated with the pathological type and molecular subtype classification of MB patients. WDR82 was a direct target of miR-155-3p. miR-155-3p was packaged into M2-Exo. miR-155-3p-enriched M2-Exo promoted the progression of Daoy cells. miR-155-3p-enriched M2-Exo promoted in vivo tumorigenesis.ConclusionThe study highlights that miR-155-3p-loaded M2-Exo enhances the growth of MB cells via down-regulating WDR82, which might provide a deep insight into MB mechanism.

【 授权许可】

CC BY