Environmental spreading of clinically relevant carbapenem-resistant gram-negative bacilli: the occurrence of blaKPC-or-NDM strains relates to local hospital activities

BMC Microbiology

Wildo Navegantes de Araújo¹  Alex Leite Pereira¹  Pâmela Maria de Oliveira¹  Rodrigo Haddad¹  Thaís Alves da Costa Lamounier¹  Everton Giovanni Alves²  Glaura Regina de Castro e Caldo Lima²  Célio Faria-Junior² 

[1] Campus of Ceilândia, University of Brasília. Centro Metropolitano, Conjunto A, Ceilândia Sul, CEP: 72220-275, Brasília, DF, Brazil;Central Laboratory for Public Health (LACEN-DF), SGAN 601, Asa Norte, CEP: 70830-010, Brasília, DF, Brazil;

关键词: Carbapenem resistance;    Sewage treatment;    Surveillance;   

DOI  :  10.1186/s12866-021-02400-1

来源: Springer

【 摘 要 】

BackgroundAquatic matrices impacted by sewage may shelter carbapenem-resistant (CR) Gram-negative bacilli (GNB) harboring resistance genes of public health concern. In this study, sewage treatment plants (STPs) servicing well-defined catchment areas were surveyed for the presence of CR-GNB bearing carbapenemase genes (blaKPC or blaNDM).ResultsA total of 325 CR-GNB were recovered from raw (RS) and treated (TS) sewage samples as well as from water body spots upstream (UW) and downstream (DW) from STPs. Klebsiella-Enterobacter (KE) group amounted to 116 isolates (35.7%). CR-KE isolates were recovered from TS, DW (35.7%) and RS samples (44.2%) (p = 0.001); but not from UW samples. KE isolates represented 65.8% of all blaKPC or blaNDM positive strains. The frequency of blaKPC-or-NDM strains was positively associated with the occurrence of district hospitals located near STPs, as well as with the number of hospitalizations and of sewer connections serviced by the STPs. blaKPC-or-NDM strains were recovered from ST samples in 7 out of 14 STPs, including four tertiary-level STPs; and from 6 out of 13 DW spots whose RS samples also had blaKPC-or-NDM strains.ConclusionsClinically relevant GNB bearing blaKPC-or-NDM resist sewage treatments and spread into environmental aquatic matrices mainly from STPs impacted by hospital activities.

【 授权许可】

CC BY