Transcriptional analysis of Bemisia tabaci MEAM1 cryptic species under the selection pressure of neonicotinoids imidacloprid, acetamiprid and thiamethoxam

BMC Genomics

Qian Cao¹  De Ying Ma²  Rui Xingyue Zhang²  Cheng Song Zhou²  Huan Huan Lv³  Xiao Hu Guo³ 

[1] Agricultural Product Inspection and Test Center, 99 Wuyi East Road, 831100, Changji, China;Engineering Research Centre of Cotton, Ministry of Education /College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, 830052, Urumqi, China;Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Universities of the Xinjiang Uygur Autonomous Region, 311 Nongda East Road, 830052, Urumqi, China;

关键词: Neonicotinoids;    Bemisia tabaci;    Resistance;   

DOI  :  10.1186/s12864-021-08241-6

来源: Springer

【 摘 要 】

BackgroundNeonicotinoids are widely applied in the control of the destructive agricultural pest Bemisia tabaci, and resistance against these chemicals has become a common, severe problem in the control of whiteflies. To investigate the molecular mechanism underlying resistance against nenonicotinoids in whiteflies, RNA-seq technology was applied, and the variation in the transcriptomic profiles of susceptible whiteflies and whiteflies selected by imidacloprid, acetamiprid and thiamethoxam treatment was characterized.ResultsA total of 90.86 GB of clean sequence data were obtained from the 4 transcriptomes. Among the 16,069 assembled genes, 584, 110 and 147 genes were upregulated in the imidacloprid-selected strain (IMI), acetamiprid-selected strain (ACE), and thiamethoxam (THI)-selected strain, respectively, relative to the susceptible strain. Detoxification-related genes including P450s, cuticle protein genes, GSTs, UGTs and molecular chaperone HSP70s were overexpressed in the selected resistant strains, especially in the IMI strain. Five genes were downregulated in all three selected resistant strains, including 2 UDP-glucuronosyltransferase 2B18-like genes (LOC 109030370 and LOC 109032577).ConclusionsTen generations of selection with the three neonicotinoids induced different resistance levels and gene expression profiles, mainly involving cuticle protein and P450 genes, in the three selected resistant whitefly strains. The results provide a reference for research on resistance and cross-resistance against neonicotinoids in B. tabaci.

【 授权许可】

CC BY